摘要
Objective To screen and identify genetic loci affecting the active zone formation in C. elegans. Methods A SYD-2：：GFP reporter was constructed and used as an active zone marker for forward genetic screen to identify genetic loci affecting the active zone formation. Results Eight isolated mutant alleles were characterized from 15,000 haploid genomes. The SYD-2：：GFP phenotypes of these mutants are mainly reflected as the changes of number, morphology, distribution of puncta and the gaps appearance. Some mutants also exhibit visible behavioral or physical phenotypes, and aldicarb resistant or sensitive phenotypes. Conclusion These mutants provide the opportunity for further systematic research on the active zone formation and the neurotransmission.
目的筛选与鉴定线虫中影响神经突触活动区形成的遗传位点。方法我们通过构建SYD-2::GFP转基因动物作为活动区的标记进行正向遗传筛选,以鉴定影响活动区形成的系列遗传位点。结果从15 000筛选群体中,我们分离出8个感兴趣的遗传位点。其突变体的表现型主要反映在SYD-2::GFP荧光标记数目、形态与分布的改变以及大缺口的出现。一些突变体还表现出明显的行为缺陷以及针对涕灭威(aldicarb)的抗性或者敏感性。结论这些突变体为我们进一步系统研究活动区的形成与神经递质释放的机制提供了重要前提。
