慢性乙肝患者HBVx-DNA表达与mIL-2R相关性

Relationship between expression of HBVx-DNA and mIL-2R in peripheral blood of the patients with chronic hepatitis B

目的探讨慢性乙肝、原发性肝癌患者乙肝病毒x基因(HBVx-DNA)表达水平与膜白细胞介素-2受体(mIL-2R)的相关性。方法采用聚合酶链反应检测慢性、原发性肝癌乙肝患者血清中HBVx-DNA,采用生物素-链霉亲和素(BSA)法检测患者外周血单个核细胞(PBMC)用植物血凝素(PHA)诱导前后mIL-2R表达水平。结果慢性乙肝、原发性肝癌患者HBVx-DNA阳性率分别为11.9%(16/135)和40.9%(9/22),2者相比差异有统计学意义(P<0.01)。其中慢性乙肝轻度、中度、重度、肝炎后肝硬化患者血清HBVx-DNA阳性率分别为0,20.8%(5/24),17.9%(5/28),17.1%(6/35),差异有统计学意义(P<0.01)。HBeAg(+)组HBVx-DNA阳性率为20.4%(21/103),与HBeAg(-)组相比,差异有统计学意义(P<0.05)。HBVx-DNA(+)组和HBVx-DNA(-)组PHA诱导前后mIL-2R表达水平分别为(2.53±1.08)%,(26.47±4.26)%和(3.34±1.23)%,(29.25±4.71)%,2者相比差异均有统计学意义(P<0.01)。肝活组织检查,原发性肝癌患者HBVx-DNA阳性率达50%(3/6),明显高于慢性乙肝和肝炎后肝硬化患者。结论慢性乙肝患者体内有HBVx-DNA存在,以中度、重度及肝炎后肝硬化患者较多见。原发性肝癌患者体内HBVx-DNA表达水平均最高,其与宿主细胞整合是导致原发性肝癌发生的重要原因。HBVx-DNA(+)患者T细胞活化功能进一步降低,可能是诱导原发性肝癌发生的另一重要原因。HBVx-DNA检测对原发性肝癌早期发现和辅助诊断具有重要意义。

Objective To study the relationship between the expression of HBVx - DNA in serum and the level of membrane interleukin-2 receptor （mIL- 2R） on surface of peripheral blood mononuclear cells （PBMC） of the patients with chronic hepatitis B and primary hepatoma. Methods The HBVx - DNA in serum and the level of mIL - 2R before and after inducement of PHA were detected by PCR and biotin - streptavidin （BSA）, respectively. Results The total positive rates of HBVx- DNA in serum of the patients with chronic hepatitis B and primary hepatoma were 11.9 % （ 16/135 ）, 40.9 % （ 9/ 22）, respectively. There was high significantly difference in two groups （ P 〈 0.01 ）. The positive rates of HBVx - DNA in serum of the patients with chronic hepatitis （light）, chronic hepatitis （moderate）, chronic hepatitis （severe）, cirrhosis were 0 %, 20.8 % （5/24）, 17.9 % （5/28）, 17.1% （6/35）, respectively. There was high significandy difference among these groups （P〈 0.01 ）. The total positive rates of HBVx- DNA in serum of group with HBeAg（ ± ） was 20.4 % （21/103）. There was higher significantly difference in group with HBeAg（ ± ） than that in group with HBeAg（ - ） （ P 〈 0.05 ）. The levels of mIL -2R before and after inducement of PHA on surface of PBMC of group with HBVx- DNA（ ± ） and HBVx- DNA（ - ） were （2.53 ± 1.08） %, （ 26.47 ± 4.26） % and （3.34 ± 1.23） %, （ 29.25 ± 4.71 ） %, respectively. There was higher signifi- cantly difference in the two groups （P〈 0.01 ）. The results of liver biopsy showed that the highest level of HBVx - DNA in tissue of hepatoma. Conclusion HBVx - DNA can be existent in the patients with chronic hepatitis B and is more common in chronic hepatitis B （moderate, severe） and cirrhosis. The positive rate of HBVx- DNA in serum of the patients with primary hepatoma was 50 % （3/6） and was higher than that of patients with chronic hepatitis B （moderate, severe） and cirrhosis. The main reason of primary hepatoma is associated with HBVx - DNA integrated with chromosome of host cells. The other important reason is that the low active T cells is more common in peripheral blood of the patients with HBVx - DNA（ ± ）. It is high significant to detect the level of HBVx - DNA in serum and PBMC for early discovery and assistant diagnosis of primary hepatoma.