哈尔滨男性LRP5、VDR基因多态性与骨密度关系

Relationship between LRP5 and VDR genes polymorphism and bone mineral density in Harbin men

目的探讨哈尔滨男性居民低密度脂蛋白受体相关蛋白5（LRP5）基因Q89R、A1330V和维生素D受体（VDR）基因FokⅠ多态性与骨密度关系。方法选取哈尔滨市132例无亲缘关系20～55岁汉族男性作为研究对象，检测其膳食钙的摄入量，测定血清生化指标、激素水平和骨密度（双能X线）。应用PCR-限制性片段长度多态性（PCR—RFLP）法检测LRP5基因Q89R、A1330V、VDR基因FokⅠ多态性。结果不同基因型之间血清生化各项指标、各项激素水平差异均无统计学意义。Q89R基因型与股骨颈（NECK）校正后的骨密度呈显著相关（P=0．047），与Ward三角（WARD）和大转子区（TROCH）骨密度有相关趋势（P值分别为0．073和0．081），而FokⅠ和A1330V基因多态性与骨密度无相关性。未发现这3个位点的基因型对骨密度有联合作用。结论LRP5基因Q89R多态性可能影响哈尔滨市男性NECK部位的骨密度。

Objective To investigate the association of LRP5 gene Q89R, A1330V and VDR gene Fok Ⅰ polymorphism with bone mineral density（BMD） in Harbin Men. Methods 132 unrelated healthy men of Han nationality, aged 20 - 55 from Harbin, were selected. Dietary calcium intakes, serum biochemistry, serum hormones, bone mineral density were meastared by dual energy X-ray, densitometry were assessed in all subjects. The polymorphisrns of LRP5 gene and VDR gene were determined using PCR-RFLP. Results It failed to find that the values of serum biochemistry and serum hormones were different statistically among genotypes. There was a significant association between Q89R and adjusted bone mineral density （BMD） at Femoral neck（NECK） （ P = 0.047 ）, and a marginal association was observed at Ward＇ s triangle （WARD） （ P = 0. 073）and trochanter（TROCH）（ P = 0. 081 ）. Neither Fok Ⅰ nor A1330V genotypes was significant association with BMD after adjusting other factors at any skeleton sites. There were not interactions for BMD among three genotypes. Conclusion LRP5 gene Q89R polymorphism influence on BMD at NECK in Harbin men.