霍乱毒素B亚单位胰岛素混合蛋白抗糖尿病作用

Effect of admixture of cholera toxoid B and insulin on spontaneous autoimmune diabetes

目的观察口服不同剂量霍乱毒紊B亚单位（CrB）与胰岛紊（insulin）混合蛋白疫苗的免疫耐受及预防1型糖尿病作用，为探讨社区糖尿病干预方案提供依据。方法3周龄雌性NOD小鼠60只，随机分为磷酸缓冲液（PBs）阴性对照、1mg胰岛紊阳性对照、CTB＋insulin100μg，CTB＋insulin 1mg等4组。从4周龄起，每周灌胃2次～12周龄；12周龄起，每周1次动态监测小鼠尿糖、血糖，至26周龄，观察糖尿病的发生，比较各组糖尿病发病率。结果与磷酸缓冲液（PBs）组比较，CTB＋insulin100μg。CTB＋insulin 1mg能够减少NOD小鼠糖尿病的发生，降低小鼠糖尿病的累积发病率。至鼠龄26周时，PBS组、CTB＋insulin100μg，CTB＋insulin1mg组和1mg胰岛素组的累积发病率分别为100％，60％，60％和40％，与阴性对照组比较，其他3组的累积发病率差异均有统计学意义（均P〈0．05）；2个试验组与阳性对照组的累积生存率及生存分布的差异无统计学意义。结论CTB＋insulin混合蛋白疫苗能够降低NOD小鼠糖尿病累积发病率，提高小鼠生存率；CTB＋insulin1mg组的预防作用优于100μg组。

Objective To observe the diabetes prevention effects of oral administration of the admixtures of insulin and cholera toxin B subunit（CTB）on female nonobese diabetic mice, and to offer evidence for constructing diabetes administrative project in community. Methods 40 3 - weeks - old female NOD mice were divided randomly into four groups. Oral administration persisted from 4 - weeks - old to 12 - weeks - old. The whole observation lasted to 26 - weeks - old. Glycosuria and glycemia were measured from 12 weeks to 26 weeks. The cumulative incidence rate were compared to estimate the effect of oral administration. Results Oral administration of CTB ＋ insulin 100μg, CTB ＋ insulin lmg and insulin 1mg to female NOD mice significantly suppressed incidence of diabetes than PBS group. The cumulative incidence rate of PBS, CTB ＋ insulin 100 μg, CTB ＋ insulin l mg and insulin 1 mg were 100 %, 60 %, 60 %, 40 % （P 〈 0.05）. And two examination groups had no significant difference with the insulin 1mg group. Conclusion Oral administration of CTB ＋ insulin 100 μg, CTB ＋ insulin ling to female NOD mice significantly can suppress incidence of diabetes and enhance the diabetes prevention effect than PBS group.