纳米氧化铁对细胞膜流动性影响及其遗传毒性

Effect of hematite nanoparticles on membrane fluidity and experiments on genotoxicology

目的从细胞膜流动性和胞内遗传物质损伤程度对纳米氧化铁的氧化作用进行检测与评价。方法以1,6-二苯基-1,3,5-己三烯(DPH)作为荧光标记,通过荧光分光光度计检测荧光强度变化,计算细胞膜的流动度;单细胞凝胶电泳(SCGE)检测胞内遗传物质在DNA水平的氧化损伤情况。结果仅在128μg/ml剂量水平观察到细胞膜流动性的降低,但8,32,128μg/ml 3个剂量组均未引起细胞内DNA损伤。结论一定剂量的纳米氧化铁(Fe2O3)颗粒可引起细胞膜结构的氧化损伤,表现为膜流动性的降低。但各剂量组均未观察到纳米Fe2O3的遗传毒性效应。

Objective To investigate the oxidative injury of hematite nanoparticles from the point of influence on membrane fluidity and its genotoxicology effects in vitro. Methods To determinate the fluorescent intensity by spectroflurophoto meter with 1, 6-diphenyl-1, 3, 5-hexatriene（DPH）as a fluoreseein mark, thus to calculate the value of fluidity degree according to the specific formula with fluorescent intensity data. To observe the damage of hematite nanoparticles to DNA by single-cell gel eleetrophoresis（SCGE）test in vitro. Results Decrease of membrane fluidity was found in group of dose 128μg/ml, however no obvious damage to DNA observed at every dose level of 8, 32,128 μg/ml. Conclusion Under the condition of our test, it is demonstrated from the experiment results in vitro that Fe2O3 nanoparticles may induce the oxidative stress in cells and consequently lead to lipid peroxidation on membrane, while no obvious damage to genetic materials in cells.