细胞打印的可行性研究

The research of feasibility of computer-aided jet-based cell printing

目的:探讨初步建立一个细胞打印系统,进行细胞无损伤打印的可行性。方法:常规培养人类宫颈癌细胞株Hela细胞,收获后做成细胞悬液代替喷墨封装入墨盒,改装喷墨打印机进行细胞打印,检测打印后细胞的活性,并与未经打印组细胞对照。结果:经过喷墨打印后细胞能够快速、精确的定位于目标区域,并且能够维持正常细胞形态、保持生物活力并继续生长、分裂增值。结论:细胞无损伤打印是可行的,为进一步的器官打印研究提供依据、探索方法。

Objective：To study the feasibility of compute-aided jet-based cell printing and set up a model to evaluate the efficiency of computer-aided jet-based cell printing. Methods： A computer-aided jet-based cell printing system including cell culture and Ink-jet printing was established. Hela cell was be used as printing stuff. A refited Epson Color480 Ink-jet printer as be the bio-printer. As jet-black, the cultured Hela cells were collected and be enclosed in ink-box and printed by bio-printer. Then the printed cells was collected and cultured again as experimental group. Another cultured cells that not be printed as control group . The control and experiment are all be cultured in same condition. The observed index includes cell counting, morphological change. Results：Cell can be placed accurately and fleetly on the printing substrate film by jet-printer. Most of the printed cells（〉90%） were survived healthily. They can be cultured again easily and the biological characteristic such as shape and cell division is same as the cells not be printed. Conclusions：The computer-aided jet-based cell printing system don＇t significantly injury cell and the method is feasible. That means organ printing maybe feasibility.