摘要
gus基因导入烟草获得转基因植株及其组织特异性表达邓晓梅;周根余(上海市农业科学院农业生物技术研究中心上海201106;上海师范大学生物系,上海200234)关键词烟草,共培养转化;gus基因;组织特异性表达烟草是我国重要的经济作物之一,烟草花叶病毒...
Agrobacterium tumefaciens strain A208SE harbouring a virus plasmid pTi37SE and binary vector PROA93 was used for the transformation of tobacco leafdiscs.Transgenic tobacco plaaes were obtained after optimizing different conditions of theleaf disc transformation including the establishment of high frequency plantletsdifferentiation from cultured tobacco leaf discs.The gus tissue-specific expression waschecked by histochemical method and the results showed that the gus gene regulated byCaMV35 s promoter expressed tissue-specific in transgenic tabaccos.
出处
《上海农业学报》
CSCD
1996年第4期85-87,共3页
Acta Agriculturae Shanghai
关键词
烟草
转化
基因
组织特异性表达
Tobacco
Leaf disc transformation
Tissue-specific expression