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gus基因导入烟草获得转基因植株及其组织特异性表达 被引量：2

TRANSGENIC TOBACCO PLANTS OBTAINED BY INTRODUCTION OF EXOGENOUS GUS REPORT GENE AND TISSUE SPECIFIC EXPRESSION OF GUS
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摘要 ｇｕｓ基因导入烟草获得转基因植株及其组织特异性表达邓晓梅；周根余（上海市农业科学院农业生物技术研究中心上海２０１１０６；上海师范大学生物系，上海２００２３４）关键词烟草，共培养转化；ｇｕｓ基因；组织特异性表达烟草是我国重要的经济作物之一，烟草花叶病毒... Agrobacterium tumefaciens strain A208SE harbouring a virus plasmid pTi37SE and binary vector PROA93 was used for the transformation of tobacco leafdiscs.Transgenic tobacco plaaes were obtained after optimizing different conditions of theleaf disc transformation including the establishment of high frequency plantletsdifferentiation from cultured tobacco leaf discs.The gus tissue-specific expression waschecked by histochemical method and the results showed that the gus gene regulated byCaMV35 s promoter expressed tissue-specific in transgenic tabaccos.
作者 邓晓梅 周根余
机构地区 上海市农业科学院农业生物技术研究中心
出处 《上海农业学报》 CSCD 1996年第4期85-87,共3页 Acta Agriculturae Shanghai
关键词 烟草 转化 基因 组织特异性表达 Tobacco Leaf disc transformation Tissue-specific expression
分类号 S572.032 [农业科学—烟草工业]
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上海农业学报
1996年 第4期

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