除草剂苯噻草胺污染对大肠杆菌抗氧化酶和ATP酶的影响

Affection of Herbicide Mefenacet Pollution to Antioxidant Enzyme and ATPase of Escherichia coli

研究了暴露于苯噻草胺的大肠杆菌中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和ATP酶活性在短期内的变化.结果表明,苯噻草胺对细菌的SOD活性诱导作用明显,尤其对处于对数生长期的细菌所产生的影响大于稳定生长期的细菌;苯噻草胺对CAT也有强烈的诱导作用,对处于不同生长阶段细菌的影响类似于对SOD的影响结果;苯噻草胺能激活ATP酶,但与细菌生长阶段的关系较小.本研究结果表明,抗氧化酶和ATP酶相结合作为该类化合物对土壤污染胁迫的敏感生物指示物具有一定的可行性.

The research is conducted on the short-term activities found in superoxide dismutase （SOD）, catalase （CAT） and ATPase of Eschierichia coli which are all exposed to mefenacet. The results show that mefenacet obviously induces SOD activity in E. coli. For those SOD in exponential phase, mefenacet affects them more greatly than it affects those in stable phase. Mefenacet also exerts great inducement to CAT activity, and affects the bacterium in various growth phase in the similar fashion it does to SOD. Mefenacet can activate ATPase; but shows less correlation to bacterium growth phase. The research shows that it is feasible to use antioxidant enzyme combined with ATPase as a biomarker for pollution menace posed by these chemicals in soil environment.