滋阴泻火方对性早熟大鼠血清类胰岛素样生长因子-Ⅰ水平的影响

Effects of Chinese herbal medicine for nourishing Yin and purging Fire on IGF-Ⅰin central precocious puberty model of female rats

目的探讨滋阴泻火方对性早熟模型大鼠血清类胰岛素样生长因子-Ⅰ(IGF-Ⅰ)水平的影响。方法26日龄雌性SD大鼠36只,随机分成6组,即正常对照组、性早熟模型组、亮丙瑞林组及中药大、中、小剂量组。除正常对照组外,其余各组均采用N-甲基-DL-天冬氨酸(NMA)40 mg.kg-1皮下注射建立性早熟模型,其中亮丙瑞林组用亮丙瑞林干预,中药各组用不同剂量的滋阴泻火方干预,观察不同干预措施对性早熟模型大鼠卵巢指数、子宫指数及血清IGF-Ⅰ水平的影响。结果性早熟模型组和中药小剂量组的卵巢指数[分别为(6.37±1.61)×10-4、(5.10±2.59)×10-4]和子宫指数[分别为(8.91±2.32)×10-4、(7.86±1.98)×10-4]均显著高于其他各组(P<0.05);亮丙瑞林组、中药大剂量组和中药中剂量组卵巢指数和子宫指数与正常对照组相比均无统计学差异(P>0.05)。与正常对照组比较,性早熟模型组的血清IGF-Ⅰ水平显著升高[(327.08±46.15)vs(239.40±61.92)m IU.m l-1,P<0.05],而4个干预组均无显著性差异(P>0.05);与性早熟模型组比较,中药大剂量组[(218.83±77.06)m IU.m l-1]和中剂量组[(207.09±53.00)m IU.m l-1]的血清IGF-Ⅰ水平显著降低(P<0.05),中药小剂量组无显著性差异(P>0.05);中药各剂量组与亮丙瑞林组比较无显著性差异(P>0.05)。结论滋阴泻火方大、中剂量能降低性早熟模型大鼠的血清IGF-Ⅰ的水平。

Objective To elucidate the effect of Chinese herbal medicine （CHM） for nourishing Yin and purging Fire on insulin-like grouth factor Ⅰ （IGF- Ⅰ） in the N-methyl-DL-aspartate （NMA） induced central precocious puberty （CPP） model of female rats. Methods Thiry-six 26-day-old SD female rats were randomly devided into the normal control group, the model group, the leuprorelin group, the high-dose of CHM group, the middle-dose of CHM group,and the low-dose of CHM group. All Rats, except in the normal group, were subcutaneously administered NMA. In the meantime, the leuprorelin group, high-dose, middle-dose and low-dose of CHM groups received gavage of leuprorelin, or different dose of CHM once a day respectively. The parameters were analyzed, which including the concentration of serum IGF- Ⅰ , the ovary index and the uterus index. Results The ovary index and the uterus index in model group and the low-dose of CHM group were much higher than those in other groups （P 〈 0. 05 ）. The ovary index and the uterus index in leuprorelin group, high-dose and middle-dose of CHM groups had no differences from the normal group （ P 〉 0. 05 ）. The concentration of serum IGF- Ⅰ in model group were much higher than that in the normal group（P 〈0. 05）. The concentration of serum IGF-Ⅰ in high-dose and middle-dose of CHM groups were much lower than that in the model group（P 〈0. 05） The concentration of serum IGF-Ⅰ in all CHM groups had no differences from the leuprorelin group（ P 〈 0.05 ）. Conclusions CHM for nourishing Yin and purging Fire may reduce the concentration of serum IGF-Ⅰ in CPP model of female rats.