摘要
采用体外液体培养和琼脂半固体培养方法,观察WEHL-3细胞的生长特点。结果表明,种入的细胞浓度越高,细胞增殖越快;WEHI-3细胞条件培养液对WEHI-3细胞的生长、集落形成及小鼠粒-单系造血祖细胞集落(CFU-GM)产率均有明显的刺激作用(P<0.01)。提示WEHI-3条件培养液具有GM-CSF活性。
The liquid assay and the semisolid agar assay were used to investigate the growth character-istics of WEHI一3cells. It was demonstrated that multiplication rate of the ells with 104 cells·ml-1 in concentration was greater than those with 103 cells·ml-1 in concentration(P<0. 05),and the number of WEHI-3 cells colonies formed increased significantey when high concentrationof WEHI一3 cells were plated(p<0.05).WEHI-3 conditioned medium has evident stimulatingeffects on the proliferation of WEHI- 3 cells,and on the formation of murine CFU-GM colonies. These results suggest that WEHI-3 cells can produce GM-CSF activity and by the effects of suchactivity WEHO-3 cells promote their self一growth.This study provides essential data for usingWEHI-3 cells as cell model for further investigation.
出处
《湖南医科大学学报》
CSCD
1996年第3期233-235,共3页
Bulletin of Hunan Medical University
基金
国家自然科学基金