鼻息肉中IgE及GM-CSF的表达与嗜酸性粒细胞浸润的相关性研究

Study on the correlation of IgE and GM-CSF contents with eosinophil accumulation in nasal poly

目的:探讨鼻息肉组织中免疫球蛋白E(IgE)和粒细胞巨噬细胞集落刺激因子(GM-CSF)的表达对嗜酸性粒细胞浸润聚集的作用及二者的关系及意义。方法:采用双抗体夹心酶联免疫吸附测定法,分别检测30例鼻息肉标本和11例鼻中隔手术患者下鼻甲黏膜标本组织匀浆中IgE和GM-CSF水平,同时观测两组组织中嗜酸性粒细胞的浸润程度。结果:鼻息肉组织中IgE、GM-CSF水平及嗜酸性粒细胞数均明显高于对照组,差异有显著性意义(P均<0.01),且二者水平分别与鼻息肉组织中嗜酸性粒细胞数呈正相关(r分别为0.68和0.62,P<0.01),且鼻息肉中IgE与GM-CSF水平呈正相关(r=0.54,P<0.01)。结论:鼻息肉是以嗜酸性粒细胞浸润为特征的疾病过程;IgE及GM-CSF则对鼻息肉中嗜酸性粒细胞浸润发挥重要作用;IgE介导的I型变态反应在鼻息肉的形成和发展中占有重要地位。

Objective：The purpose is to study the effect of IgE,GM-CSF to eosinophil accumulation in the nasal polyps and find the relationship between IgE and GM-CSF. Methods： Thirty mucosa constitution samples of nasal polyps and eleven inferior nasal concha were observed. The concentration of IgE and GM-CSF was measured with ELISA techique in homogenized polyp tissue and in control mucosa, and the number of eosinophils were counted in the same tissues. Results：The statistical analysis showed that the level of IgE, GM-CSF and eosinophils have significant different between the nasal polyps and control group （P 〈0.01）. There was positive correlation between IgE/GM-CSF and the number of eosinophils in the nasal polyps group （r =0.68, r =0.62, P 〈0.01 ）. The correlation between concenstration of IgE and GM-CSF was also significant in nasal polyps group（r =0.54, P 〈 0.01 ）. Conlusion： Nasal polyps is characterized by eosinophil accumulation. IgE and GM-CSF may be contributed to the recruitment of eosinophils in nasal polyps. Type-1 allergy mediated by IgE played an important role in the pathogenesis of nasal polyps.