基质金属蛋白酶MMP20与喉癌的浸润和转移

Matrix mettallo-proteinase 20 in laryngeal squamous cell carcinoma

目的探讨基质金属蛋白酶MMP20与喉癌浸润和转移的关系。方法用RT-PCR和免疫组化的方法,研究MMP20在喉癌组织和其相邻的正常黏膜组织的差异表达量,分析其表达与喉癌浸润和转移的关系。数据分析借助SPSS10.0软件完成。结果36例配对标本中MMP20基因在31例喉癌组织中的表达与其相应的正常黏膜组织差异表达不明显,而在5例喉癌组织中差异表达明显;MMP20蛋白绝大多数在癌巢细胞浆内表达(占93.2%,68/73),少数主要在细胞核内表达(占6.8%,5/73),MMP20蛋白在大多数喉癌组织中的表达明显高于其相应的正常黏膜组织;有淋巴结转移病例中,MMP20蛋白表达差异明显的病例数高于没有淋巴结转移者(P=0.023);5例MMP20蛋白喉癌组织细胞核内表达中有4例与淋巴结转移有关。结论MMP20蛋白在喉癌的淋巴结转移中起着重要的作用;MMP20蛋白的过度表达可作为喉癌淋巴结转移的标志。

OBJECTIVE To investigate the role of expression of matrix mettalloproteinase 20（MMP20） in invasion and metastasis of the laryngeal carcinoma. METHODS The gene expression MMP20 in fresh laryngeal cancer tissues and their corresponding normal mucosa was detected by Reverse transcriptionpolymerase chain reaction （RT-PCR）. The expression of MMP20 protein in paraffin-embedded tissues was determined by immunohistochemical method. All statistical analyses were carried out with SPSS version 10.0. RESULTS In the 36 matched specimens,MMP20 gene expression was much higher in tumor tissues than that in corresponding normal tissues in 5 cases,whereas in other 31 cases,no significant difference was observed between the cancer tissues and the corresponding normal tissues. MMP20 protein was located predominantly in the tumor cell cytoplasm in most cases,in nucleus in a few cases. MMP20 protein was over-expressed in most of the laryngeal cancer tissues. In contrast, there was weakly or not expressed in corresponding normal tissues. A significant correlation was found between MMP20 protein expression and lymph node metastases（P=0.023）. Four of 5 cases of MMP20 protein expressed in nucleus were related to the invasion and metastasis of laryngeal carcinomas.CONCLUSION The protein MMP20 may play a role in laryngeal cancer invasion in a certain extent,but may play an important role in lymph node metastasis of laryngeal cancer. The over-expression of MMP20 protein may be a marker for lymph node metastasis oflaryngeal cancer.