白细胞介素-6基因多态性与尘肺的相关性

Relationship between genetic polymorphism of interleukin-6 and pnemnoconiosis

目的研究白细胞介素-6(IL-6)(-634C/G)基因多态性与尘肺的关系。方法选择确诊的104例Ⅰ期男性矽肺患者66例(矽肺组)、煤工尘肺患者38例(煤工尘肺组)为研究对象,以接触同性质粉尘、非尘肺的122名男性接触矽尘工人77例(矽尘组)、接触煤尘工人45例(煤尘组)为对照；采集外周静脉血,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测IL-6(-634C/G)基因多态性。结果IL-6(-634C/G)基因型(CC、CG、GG)在矽肺组、矽尘组、煤工尘肺组和煤尘组分布频率分别为66．7%、19．7%、13．6%,42．9%、42．9%、14．2%,73．7%、18．4%、7．9%和51．1%、35．65、13．3%。成组分析和1∶1配对分析中均发现IL-6(-634C/G)基因型分布频率在矽肺组和矽尘组间差异有统计学意义(P＜0．05)。结论IL-6(-634C/G)基因多态性与矽肺发病相关。

Objective To explore the relationship between interleukin-6（IL-6）（-634C/G） genetic polymorphisms and the pneumoconiosis. Methods A total of 104 male stage I pneumoconiosis patients diagnosed by the Pneumoconiosis Diagnosis Expert Panel according to the Chinese National Diagnosis Criteria of Pneumoconiosis （GBZ 70-2002） were selected. The pneumoconiosis comprised 66 silicosis and 38 coal worker＇ pneumoconiosis （CWP）. A total of 122 workers exposed to same dusts as the patients but without pneumoconiosis including 77 exposed to silica dusts and 45 to coal dusts were selected. The patients and the controls had the same dust exposure history. The peripheral venous blood was drawn from each subject. The IL-6（-634C/G） genetic polymorphisms were detected by polymerase chain reaction restriction fragment length polymorphisms（PCR-RFLP） techniques. Results The frequencies of IL-6（-634C/G）（CC,CG and GG） genotypes were 66.7%, 19.7% and 13.6% in silicosis group,42.9%,42.9% and 14.2% in silica dust exposure group, 73.7%, 18.4% and 7,9% in CWP group, 51.1% ,35.6% and 13.3% in coal dust exposure group respectively.The statistical analysis indicated that there was significant difference in the frequencies of IL-6（-634C/G） （ CC, CG and GG） genotypes between silicosis patients and workers exposed to silica dusts（ P 〈 0.05）. Conclusion IL-6 （-634 C/G） genetic polymorphisms might play a role in the occurrence of silicosis.