牛磺酸对染矽尘大鼠肺组织Ⅰ和Ⅲ型胶原合成及比例的影响

Effects of taurine on typeⅠandⅢcollagen expression in rats lung exposed to silica

目的探讨牛磺酸对染矽尘大鼠肺组织不同时相Ⅰ、Ⅲ型胶原合成及比例的影响。方法将Wistar大鼠144只随机分为对照组(生理盐水,普饲)、矽肺组(普饲)和牛磺酸组(牛磺酸饲料)给予气管内注入1ml矽尘混悬液(50mg/ml),每组48只。分别在第1、3、7、14、21、28天时,用高效液相色谱法测定其血浆中牛磺酸含量；取肺组织制作石蜡切片,天狼猩红法染色,偏振光显微镜观察各组动物肺组织Ⅰ、Ⅲ型胶原的合成及比例的变化,图像分析系统对其进行定量分析。结果第7、14、21和28天,矽尘组Ⅰ型胶原含量分别比对照组增加3,84%、3．77%、3．73%、9．83%,差异有统计学意义(P＜0．01)。第7、21和28天时,牛磺酸组Ⅰ型胶原含量分别比矽尘组降低2．39%、1．62%和7．13%；与对照组比较,第14、21和28天Ⅰ型胶原含量分别增加2．12%、2．11%和2．70%,差异均有统计学意义(P＜0．05或P＜0．01)。第28天时,牛磺酸组Ⅲ型胶原含量比矽尘组减少2．62%,差异有统计学意义(P＜0．05)。矽尘组Ⅰ/Ⅲ型胶原比例第7天开始增加,第28天时达到最大比值1．87,牛磺酸组Ⅰ/Ⅲ型胶原比例从第7天开始增加,且增加幅度高于矽尘组,第28天时增加幅度低于矽尘组。结论牛磺酸对矽尘诱导大鼠肺组织不同时相的Ⅰ、Ⅲ型胶原合成抑制作用不同。

Objective To evaluate the effects of taurine in diet on the expression of type Ⅰ and Ⅲ collagen and collagen ratio at different time points in rats lung by image process technology. Methods Wistar rats were randomly divided into three groups： the saline instilled with a control diet（the saline treated group） ;silica instilled with a control diet（ the silica treated group） ; and silica instilled with a diet containing 2.5% taurine（the taurine treated group）. Animal models were established by the direct tracheal instillation of silica into rat lungs exposed surgically. The taurine concentration of serum was analyzed by means of HPLC. Paraffin embedded lung sections were stained with Sirius red. Polarization microscopy and Image Pro Plus Version 4.5 for windows were used for detecting type Ⅰ and Ⅲ collagen, Results The concentration of taurine in serum of the taurine treated group was significantly elevated compared to the saline treated and silica treated group（ P 〈 0.05 or P 〈 0.01 ）. Sirius red polarization microscopy showed that type Ⅰ and Ⅲ collagen positive area percentage were elevated in the silica treated rats compared with the saline treated group. On the 7th, 14th,21st,28th day after silica instillation type Ⅰ collagen positive area percentage was increased by 3.84,3.77,3.73,9.83 respectively（ P 〈 0.01 ）, and type Ⅲ collagen positive area percentage were elevated by a little in the silica treated rats compared with saline treated group. The taurine treatment significantly decreased elevation of silica type Ⅰ collagen positive area percentage of lung by 2.39, 1.62,7.13 at the 7th, 21st, 28th day respectively（ P 〈 0.05 or P 〈 0.01 ）, and type Ⅲ collagen positive area percentage of lung by 2.62 at the 28th day（ P 〈 0.05 ） compared with the silica treated group. The ratio of type Ⅰ to Ⅲ collagen was increased from the 7th day to 28th day after silica instillation,and reached 1.87 at the 28th day with the maximal ratio in the silica-treated group. Conclusion Treatment with taurine can effectively attenuate type Ⅰ and Ⅲ collagen expression in the rat lung induced by silica particles at different time points in our study.