酶联免疫吸附分析法测定血清中醋酸亮丙瑞林含量

Enzyme-linked immunosorbent assay of leuprorelin acetate in serum

目的:建立一种检测微量醋酸亮丙瑞林的方法。方法:使用酶联免疫吸附分析法(ELISA),测定样品中醋酸亮丙瑞林含量。结果:自制的兔抗亮丙瑞林抗血清具有良好的特异性。使用 ELISA 测定醋酸亮内瑞林的最低检出限为0.025ng·mL^(-1),精密度(日内 RSD<23.5%,日间 RSD<22.4%)良好。不同浓度醋酸亮丙瑞林的平均回收率为97.3%。含样品血清冷冻(-20℃)保存30d 内,醋酸亮丙瑞林的检测结果稳定,重复性好。结论:本实验研究建立了一种操作简单、快速,特异性好、灵密度高的醋酸亮丙瑞林检测方法。

Objective：To develop a sensitive and easy handled method for the determination of serum leuprorelin acetate. Methods ：The enzyme- linked immunosorbent assay（ELISA） was established to determine the content of leuprorelin acetate in serum. Results：The rabbit＇s antiserum displayed a high selective binding to leuprorelin. The minimum of determined leuprorelin acetate was 0. 025 ng · mL^-1 using ELISA. Precision was evaluated with the intra -assay（ RSD 〈23.5% ） and inter -assay（ RSD 〈22.4% ）. Recovery rate was 97.3%. A stable and repeatable determination was still obtained when the serum contained leuprorelin acetate was stored for 30 days at 20 ℃. Conclusion ： A new method is established for the determination of serum leuprorelin acetate with ELISA.