高效液相色谱法同时测定樟脑苯酚溶液中两组分的含量

HPLC determination of the content of camphor and phenol in camphor and phenol solution

目的:采用高效液相色谱法同时测定樟脑苯酚溶液中两组分的含量。方法:采用 Hypersil C_(18)色谱柱(4.6 mm×200mm,5μm),以甲醇-水(70:30)为流动相,流速2.0 mL·min^(-1),紫外检测器于290 nm 测定。结果:HPLC 法测定,所测成分与溶剂分离良好,樟脑、苯酚的线性范围分别为1:53～3.58 mg·mL^(-1)(r=1.0000)和0.74～1.73 mg·mL^(-1)(r=0.9997),样品溶液在24 h 内稳定,总回收率(n=9)分别为99.9%与100.1%。结论:本法简便、快速,结果准确、可靠,重现性好。

Objective：To determine the content of camphor and phenol in camphor and phenol solution by HPLC. Methods： The column ： Hypersil C18 column （ 4. 6 mm × 200 mm, 5 μm, ） ; The mobile phase ： Methanol - water （ 70 ： 30） ;The flow rate：2. 0 mL · min^-1 ;The detection wavelength ：290 nm. Results：The detected component and host were separated well by HPLC. The linear response ranges were 1.53 - 3.58 mg · mL^- 1 （ r=1. 0000 ） for camphor and 0.74 - 1.73 mg · mL^- 1 （ r =0. 9997） for phenol ; The sample solution is steady within 24 h. The average recoveries （ n = 9） were 99. 9% and 100. 1%, respectively. Conclusion ：This method is simple, rapid, accurate and reliable.