采用单克隆抗体快速诊断移植肾活动性巨细胞病毒感染

Rapid diagnosis of active cytomegalovirus infection after renal transplantation by monoclonal antibody staining of blood leucocytes

采用巨细胞病毒（ＣＭＶ）单克隆抗体对４４例肾移植患者外周血白细胞进行了ＣＭＶ抗原检测，同时进行了血清学ＥＬＩＳＡ检测和临床症状观察。结果显示：２８例活动性ＣＭＶ感染患者中，２６例抗原检测阳性；１６例无活动性ＣＭＶ感染患者中，仅１例抗原检测阳性；抗原阳性白细胞数与ＣＭＶ特异性的临床症状相一致。认为单克隆抗体检测外周血白细胞中ＣＭＶ抗原的方法是早期、快速诊断活动性ＣＭＶ感染的较理想方法。

In this paper, the methods of detection of cytomegalovirus (CMV)antigen by monoclonal antibody in peripheral blood were established to test CMV infection after renal transplantation. CMV-ELISA was also applied. Forty-four patients were studied during the first three months after renal transplantation. Results:CMV antigen was positive in 25 out of 28 patients who.were active CMV infection and in 1 out of 16 patients who were not active CMV infection. The numbers of CMV-Ag positive PMNLs (Polymorphonuclear leucocytes) were corrlated with CMVspecific symptoms. Conclusion: the assay described for detection of CMV antigens in blood leucocytes by monoclonal antibody is a early and rapid method for diagnosis of active CMV infection.