IL─10与IL─1ra对大鼠腹腔Mφ诱生细胞因子的影响

The immunoregulatory effects of IL 10 and IL 1 receptor antagonist on cytokine production in rat peritoneal macrophages

通过建立大鼠佐剂性关节炎（ＡＡ）动物模型，研究了ＩＬ－１０和ＩＬ－１ｒａ对正常及ＡＡ大鼠腹腔Ｍφ分泌细胞因子的调节作用，并利用分子杂交技术在基因水平上对其作用机制进行了初步探讨。结果表明：ＡＡ大鼠腹腔Ｍφ呈高度活化状态，产生大量炎症细胞因子ＩＬ－１、ＩＬ－６、ＩＬ－８和ＴＮＦ等，ＩＬ－１０和ＩＬ－１ｒａ对正常及ＡＡ大鼠腹腔Ｍφ细胞因子的产生均呈显著抑制作用，并呈剂量依赖关系。ＩＬ－１０和ＩＬ－１ｒａ具有协同抑制作用。进一步研究发现ＩＬ－１０和ＩＬ－１ｒａ能抑制ＬＰＳ诱导的腹腔ＭφＩＬ－８ｍＲＮＡ表达，表明其抑制作用发生在基因水平上。提示ＩＬ－１０和ＩＬ－１ｒａ是有效的免疫抑制剂。

In this paper we studied the immunomodulatory effects and its mechanisms of IL 10 and IL 1 receptor antagonist (IL 1ra) on cytokine production from peritoneal macrophages in normal and AA rats. The results showed that peritoneal macrophages in AA rats could secrete more substantial IL 1,IL 6,IL 8 and TNF than those in normal rats. IL 10 and IL 1ra significantly down regulated cytokine production from peritoneal macrophages in normal and AA rats, which were dose dependent. Furthermore IL 10 and IL 1ra had synergistic inhibitory effect. Dot Blot revealed that IL 10 and IL 1ra could dramatically reduced IL 8 mRNA expression from peritoneal macrophages in normal rats. In conclusion, IL 10 and IL 1ra were potent inhibitors in immunoresponses, which can provide valuble theoretical and experimental evidence for its clinical applications to anti inflammation.