姜黄素对人乳腺癌MCF-7细胞抗增殖作用的研究

Antiproliferative effect of curcumin on human breast cancer of MCF-7 cells

目的研究姜黄素对人乳腺癌MCF-7细胞增殖、细胞周期分布及基因表达情况的影响。方法MTT法测定姜黄素生物活性及姜黄素抗增殖效应、FCM分析细胞周期分布，RT-PCR技术测定bcl-2、bax、PCNAmRNA表达。结果姜黄索对MCF-7细胞的IC50值为18．5μmoL／L。各实验组的姜黄素对MCF-7细胞的生长均有抑制作用，这种抑制作用呈时间-剂量依赖方式。FCM检测结果：姜黄素20μmol／L及40μmol／L作用于MCF-7细胞24h，可阻滞细胞周期于G0／G1期，并诱导部分细胞凋产。RT—PCR测定结果：姜黄素0～80μmol／L作用MCF-7细胞24h，bcl-2、PCNAmRNA表达水平降低、baxmRNA表达水平增加。结论姜黄素可抑制MCF-7细胞生长、阻滞细胞周期于G0／G1期，对MCF-7细胞有抗增殖作用，并能诱导部分细胞凋亡，其机制可能与姜黄素抑制bcl-2、PCNAmRNA表达，促进baxmRNA表达有关。

Objective To investigate the effect of curcumin on the proliferation, cell cycle distribution and gene expression of human breast cancer MCF-7 cells. Methods MCF-7 cells were treated with 10, 20, 40, 80 μmol/L curcumin for 24, 48 or 72 h. MTT was used to assay the biologic activities of curcumin and its antiproliferative effect. FCM was used to detect cell cycle distribution and apoptosis. The expression levels of bcl- 2, bax, PCNA mRNA were analyzed by RT-PCR. Results The curcumin ICsovalue for MCF-7 cells was 18.5 μmol/L. The inhibitory effect of curcumin on cell growth was in a time-and dose-dependent manner. MCF-7 cells treated with 20 μmol/L or 40 μmol/L curcumin for 24 h were arrested in G0/G1 phase and induced apoptosis. The expression levels of bcl-2, PCNA mRNA were decreased in MCF-7 cells by 10, 20 and 40 μmol/L curcumin treatment for 24 h, and bax mRNA was increased. Conclusion Curcumin is of inhibitory effect on MCF-7 cell growth, and it can arrest MCF-7 cells in G0/G1 phase, inhibit the proliferation and induce apoptosis. Curcumin down-regulates bcl-2, PCNA mRNA expression in MCF-7 cells, but upregulates bax mRNA expression.