紫杉醇联合肿瘤坏死因子α诱导腺样囊性癌细胞凋亡的体外研究

Apoptosis of salivary adenoid cystic carcinoma cells induced by TNF-α and paclitaxel

目的探讨人肿瘤坏死因子-α(TNF-α)联合紫杉醇对人腺样囊性癌细胞系SACC-83的抑制增殖作用、作用机制以及与细胞凋亡的关系,为化疗与生物治疗联合应用治疗腺样囊性癌提供实验依据。方法将人腺样囊性癌细胞系SACC-83和对照组原代培养鼠肺成纤维细胞根据不同药物浓度分为9组,药物作用72 h。MTT法检测细胞增殖抑制率;流式细胞术检测细胞凋亡率及细胞周期改变;观察凋亡细胞形态变化。结果联合用药组的细胞增殖抑制率显著高于单独用药组(P<0.05)。L929细胞各组的细胞增殖抑制率显著低于SACC-83细胞(P<0.05)。结论TNF-α联合紫杉醇对SACC-83细胞增殖的抑制作用以及诱导凋亡具有协同作用。

Objective To explore the apoptosis mechanism of salivary adenoid cystic carcinoma cells （ SACC-83 ） induced by recombinant human tumor necrosis factor-α （rhTNF-α） and paclitaxel. Methods The SACC-83 cells were treated with rhTNF-α alone, paclitaxel alone, or combination at different doses for 72 h. Primary cultured neonatal mice spleen fibroblast cells （L929 cells） served as control. The inhibitory ratio of cell proliferation was detected by MTT. The percentage of apoptotic cells and cell cycle distribution were detected by flow cytometry. The cell morphological change of apoptosis was observed. Results The inhibition ratio of cell proliferation was significant higher single drugs groups than in either single drug groups （ P 〈 0.05 ）. The inhibitory ratio of cell proliferation of every group of L929 cell was significant lower than that of SACC-83 cell （ P 〈 0.05 ）. Conclusion TNF-α is synergetic with paclitaxel in inhibiting cell proliferation and inducing apoptosis in SACC-83 cells.