更昔洛韦对肾小管上皮细胞生长和能量代谢的影响

Effects of gancrclovior on growth and energy metabolism of renal epithelial cell

目的:观察更昔洛韦对人肾小管上皮细胞生长及能量代谢的影响。方法:分别以3种不同浓度的更昔洛韦(1μg.ml-1,10μg.ml-1,100μg.ml-1)作用人肾小管上皮细胞HKCs细胞,应用MTT比色法作生长曲线,观察更昔洛韦对肾小管上皮细胞增殖的影响。同时,应用反相高效液相色谱法检测不同浓度药物作用24 h和48 h时各组细胞内ATP含量的变化。结果:实验组与对照组比较,不同浓度更昔洛韦对肾小管上皮细胞生长无明显影响(P>0.05)。药物浓度为100μg.ml-1实验组作用48 h后,细胞ATP含量比对照组低(P<0.05),其余与对照组无显著差异(P>0.05)。结论:更昔洛韦在治疗浓度范围内,对肾小管上皮细胞生长及能量代谢无明显影响,只有达较高浓度时对细胞能量代谢才有抑制作用,可能是临床上引起肾功改变的原因之一。

Objective：To study the effects of gancrclovior（GCV） on growth and energy metabolism of renal epithelial cell, Methods： HKC strain were treated with three concentrations of GCV（lpg/ml, 10μg/ml and 100μg/ml） for 24 h and 48 h, respectively, and then the proliferation of HKC strain were detected with MTT method. The concentration of ATP in HKC strain of three treated groups was measured by high performance liquid chromatography, Results：Compared with that of the control group, the growth of HKC strain was affected by GCV at different concentrations（P〉0. 05）, The concentration of ATP in HKC strain of the group treated with 100μg/ml GCV for 48 h was lower than that of the control group（P〈0. 05） and the concentration of ATP of other two treated groups was similar to that of the control group（P〉0. 05）. Conclusion：GCV has not significant effects on the growth and energy metabolism of renal epithelial cell among its therapeutic dosage range. Only when its dosage is very high, it inhibits the energy metabolism of the renal epithelial cell, which may be one of causes inducing changes of renal function.