甲型肝炎病毒PCR检测结果与其感染性关系的研究

STUDY ON THE RELATIONSHIP BETWEEN RESULT OF PCR OF HAV AND ITS INFECTIVITY

据检测，以３００μＷ／ｃｍ２紫外线照射１０分钟，或用１％过氧化氢处理３０分钟，对甲型肝炎病毒（ＨＡＶ）感染性的灭活率为９９．９９％，但用ＰＣＲ法未检出５＇端非编码区的扩增片段；经紫外线照时２０分钟，ＨＡＶ感染性虽全部消失，但仍可检出Ｐ１区扩增片段。结果表明，ＨＡＶ感染性的有无与ＰＣＲ扩增产物检出与否无平行关系．

Experimental study was carried out on the relationship between the results of PCR of HAV(amplification fragment of 5'-untranslated region and fragment of P1 region)and its infectivity After exposure to 300 μW/cm2 UV radiation for 10 min or to 1% hydrogen peroxide for 30 min,the infectivity of HAV could still be detected,but the fragment of 5'-untranslated region could not be detected.After exposure to UV radiation for 20 min,HAV was inactivated completely,but a part of the fragments of Pl region could still be detected.The results indicate that the infectivity of HAV has no parallel relation to the detection of fragment of 5'-untranslated region and fragment of Pl region。Institute of Biological Engineering Academy of Military Medical Sciences