细胞周期蛋白p27和Cyclin D1在胃癌中的表达及其临床意义

Expression of p27Kip1 and Cyclin D1 in human gastric carcinoma and its clinical significance

目的通过研究细胞周期蛋白p27、Cyc lin D1在胃癌组织中的表达水平,探讨其与胃癌生物学行为的关系。方法免疫组化S-P法检测50例胃癌组织中p27K ip1、Cyc lin D1蛋白的表达水平。患者术前均行电子纤维镜检查,并经病理证实为胃癌。每例标本均含原发癌组织和周围正常组织,对照组周围正常组织距离癌组织5 cm以上。患者术前均未接受任何抗肿瘤治疗。结果50例胃癌中,癌组织p27K ip1蛋白阳性22例,占44.0%,与正常组织比较显著降低(P<0.001);Cyc lin D1蛋白表达阳性24例,占48.0%,与正常组织比较显著升高(P<0.001);p27K ip1蛋白表达水平与胃壁浸润深度、TNM分期、病理组织学分级、区域淋巴结转移均相关(P<0.05);而与肿瘤大小、部位、患者的年龄无显著相关性(P>0.05)。Cyc lin D1蛋白表达与病理组织学分级相关(P<0.05);p27K ip1与Cyc lin D1两者蛋白阳性表达无显著相关性(P>0.05);结论(1)p27K ip1蛋白表达可作为反映肿瘤恶性程度的指标,在一定程度上可反映胃癌病人的预后;(2)Cyc lin D1蛋白表达与胃癌病理组织学分级呈相关性,其过表达可能与胃癌的发生有关;(3)胃癌组织中p27K ip1与Cyc lin D1蛋白阳性表达两者无相关性;

Objective This study was designed to investigate the expression of p27Kipl and Cyclin DI in gastric carcinoma and their relation to biological behavior of gastric carcinoma. Methods p27Kipl and Cyclin DI expressions at protein level were determined by immunohistochemistry technique in 50 patients with gastric carcinoma. All patients were diagnosed through electron fiberscopy and confirmed by pathological inspection. Each of specimen contained both primary carcinoma tissue and normal tissue around carcinoma. Normal tissues which were more than 5cm from carcinoma tissue were collected as control specimen. All of patients had not received any antineoplaston. Results The positive rate of p27kipl expression in gastric carcinoma among 50 cases with gastric carcinoma was 44.0 % （22 cases） , and p27kipl expression was lower in gastric carcinoma than that in normal tissue. The positive rate of Cyclin DI expression in gastric carcinoma was 48.0 % （24 cases） , and Cyclin DI expression was higher in gastric carcinoma than that in normal tissue, p27Kipl expression level was correlated with histological grade, depth of invasion ,lymphnode metastasis and TNM stage（ P 〈 0. 05） , but it had no relation with the tumor size , site as well as the age of patient. Cyclin DI expression level was correlated with histological grade. The positive expression of p27Kipl has no significant correlation with the positive expression of Cyclin DI. Conclusion （ 1 ） p27Kipl may be a useful marker for tumoral malignant behavior, which reflects prognosis of human gastric cancer in some degree. （2） Cyclin D I expression level was correlated with histological grade,its hyper-expression may be related to gastric carcinogenesis. （3） The positive expression of p27Kipl has no significant correlation with the positive expression of Cyclin DI in carcinoma tissue.