摘要
Objective:To establish a new way to collect superovulated oocytes or zygotes repeatedlyfrom an individual mouse.Methods:Superovulations were induced by injection PMSG and hCG in Kunming strainmice.The ampullaes of oviduct of all anaesthetised mouse were put in a specially designed'U'sink and released.The second and third times of PMSG injection were made on the sixth day andeleventh day after the first superovulation injection.The capacity of development was examinedby in vitro culture of parthenogenesis activation oocytes.Results:Development to blastocyst stage was not significantly different between the first andsecond time collection.The percentage of blastocyst stage in CD and Sr^(++) treatment was signifi-cantly higher(P<0.05)than the oocytes treated in CB and Sr^(++).Conclusion:This method enables us to collect oocytes or zygotes repeatedly from one individ-ual mouse in an interval as short as 5 days and without influence on the quality of oocytes.
Objective： To establish a new way to collect superovulated oocytes or zygotes repeatedly from an individual mouse. Methods： Superovulations were induced by injection PMSG and hCG in Kunming strain mice. The ampullaes of oviduct of all anaesthetised mouse were put in a specially designed ＂U＂ sink and released. The second and third times of PMSG injection were made on the sixth day and eleventh day after the first superovulation injection. The capacity of development was examined by in vitro culture of parthenogenesis activation oocytes.Results： Development to blastocyst stage was not significantly different between the first and second time collection. The percentage of blastocyst stage in CD and Sr^＋＋ treatment was significantly higher （P〈0.05） than the oocytes treated in CB and Sr^＋＋. Conclusion： This method enables us to collect oocytes or zygotes repeatedly from one individual mouse in an interval as short as 5 days and without influence on the quality of oocytes.
出处
《生殖医学杂志》
CAS
2005年第B10期68-72,共5页
Journal of Reproductive Medicine
