刺五加对大鼠脑出血病理变化及β-EP的影响

Effects of acanthopanax senticosus injection on the brain tissue,beta-endorphin in rats with intracerebral hemorrhage

目的观察不同剂量的刺五加(AS)注射液对大鼠脑出血(ICH)病理变化及脑组织β-内啡肽(β-EP)含量的影响,为临床AS注射液治疗ICH提供一定的理论依据。方法采用胶原酶注入法建立大鼠ICH模型;干湿重法测定各组ICH大鼠脑组织含水量;电镜观察脑组织病理变化;放免法测定脑组织β-EP的含量。结果(1)脑含水量:用药第3、7天,大、中剂量组均显著低于对照组(P<0.01);第7天,大、中剂量组显著低于小剂量组(P<0.01),小剂量组明显低于对照组(P<0.05)。(2)电镜观察显示:第7天,对照组血肿旁脑组织普遍可见神经胶质细胞核染色质边集现象,神经细胞和神经胶质细胞线粒体显著肿胀、空泡样改变,线粒体嵴减少;中剂量组上述变化不明显。(3)脑组织β-EP含量:用药第1天,各治疗组显著高于对照组(P<0.01);第3、7天,大、中剂量组均显著低于对照组(P<0.01),大剂量组显著低于小剂量组(P<0.01)。结论(1)AS注射液能明显减轻ICH大鼠脑水肿并阻断ICH后脑细胞凋亡,对大鼠ICH有确切的治疗效果。在脑出血急性期即可开始应用AS注射液,在允许的范围内应用最大剂量能达到最佳治疗效果。(2)AS注射液可能具有β-EP受体阻断剂样作用。

Objective To explore the effects of various doses of acanthopanax senticosus （AS） injection on the pathological changes of the brain tissue,the content of beta-endorphin （β-EP） in brain tissue after intracerebral hemorrhage （ICH） ,and to provide theory reference for ICH treated by AS injection clinically. Methods ICH rats models were established by injecting eollagenase into their brain. The brain water content was determined with the wet and dry weight method ; the pathological changes of brain tissue were observed by electron-microscopy ;the content of β-EP in brain tissue were determined with radioimmunoassay. Results （ 1 ） The brain water content in the great and middle dose group were significantly lower than that in control group at the 3rd and 7th day after treatment （ P 〈0.01 ） ;that in great and middle dose group were significantly lower than that in small dose group （P 〈0. 01） ;and that in small dose group was markedly lower than that in control group （P 〈0. 05） at the 7th day. （2） Electron-microscopy showed that ehromatins gathered to the rim of nucleus in most nerve gliocytes, mitochondria were swollen, vacuoles were formed and had decreased crests in most neurocytes and nerve gliocytes around the hematoma in the eontrol group at the 7th day,but it couldn＇t be seen evidently in the middle dose group on the same time. （3） The content of β- EP in brain tissue：three treated groups were significantly higher than that in control group （P 〈0. 01）at the 1st day after treatment,but that in great and middle dose group were significantly lower than that in control group （ P 〈 0. 01 ） and that in great dose group was lower than that in small group （ P 〈 0. 01 ） at the 3rd and 7th day after treatment. Conclusions （ 1 ） AS injection can obviously relieve the brain edema and block the apoptosis of brain cells of the ICH rats having remedial effect for ICH rats with optimal therapeutic effect at the great dosage within permission. （2） AS injection may have an effect just like a blocking agent of β-EP aceeptor.