金薯叶止血合剂对ITP模型动物的治疗作用

Therapeutic Effect of Jinshuye Mixture on ITP Mouse

目的:观察金薯叶止血合剂对特发性血小板减少性紫癜(ITP)模型小鼠外周血血小板(PLT)数、骨髓巨核细胞数和巨核细胞数分类、外周血T淋巴细胞亚群变化的影响。方法:用豚鼠抗小鼠血小板血清(APS)连续注射小鼠,造成ITP小鼠模型,分别给金薯叶止血合剂(大、中、小剂量即5g生药.kg-1.d-1、2.5g生药.kg-1.d-1、1.25g生药.kg-1.d-1),正常对照组、模型对照组给予生理盐水,连续给药10d;血细胞分析仪测定PLT,显微镜法观察巨核细胞形态分类,塑料包埋法计数小鼠股骨巨核细胞数,流式细胞仪检测T淋巴细胞亚群。结果:模型小鼠外周血PLT数持续明显减低、骨髓巨核细胞数增多,其中原幼巨核细胞比例明显增高,产板巨核细胞比例明显减低,外周血CD4+CD8-细胞减低,CD8+CD4-和CD4+CD8+细胞明显增高,CD4+/CD8+比值明显降低。给予金薯叶止血合剂后PLT明显增高、巨核细胞数减少、产板巨核细胞比例明显增高,CD8+CD4-和CD4+CD8+细胞比例明显降低,CD4+/CD8+比值升高。结论:金薯叶止血合剂能提升ITP模型小鼠PLT数,提高产板巨核细胞比例,改善T淋巴细胞的失衡,达到治疗ITP的作用。

Objective： To observe the effect of Jinshuye Prescription on peripheral blood PLT, megakaryocytes, divisions of megakaryocyte, peripheral lymphocyte subgroups of idiopathic thrombocytopenic purpura （ITP） mouse model. Methods： Using APS to intraperitoneally inject into the mice to induce the ITP mice model continually, administer Jinshuye Prescription according to 3 concentrations respectively. The drug is to be given for 10 days. Then peripheral blood PLT is detected, the morphology of megakaryocyte is observed by microscope, the T lymphocyte subsets are detected by cytometer. Results：the periphral blood PLT is continually decreased, the marrow megakaryocytes increased, among which the megakaryoblast raito obviously increased, the PLT producing megakaryocytes ratio reduced obviously, CD4^＋ CD8^＋ arising, CD8^＋ CD4^-, CD4^＋CD8^＋ produced, CD4^＋/CD8^＋ ratio reduced. After Jinshuye Prescription is administered, PLT and PLT producing megakaryocyte are increased, but megakareocytes, CD8^＋CD4^- , CD4^＋ CD8^＋ reduced. Conclusion： Jinshuye Prescription is able to increase PLT, PLT producing megakaryocyte ratio, relieve T lymphocyte failure state of disbalance. All of the above indicates it may be the mechanism of Jinshuye Prescription toward treating ITP patients.