蕈样肉芽肿／Sézary综合征中Th1和Th2型细胞因子的表达特点及其意义

The Characteristics of expression of Th_1 & Th_2 cell-medicated cytokine profile and its significance in MF/SS

采用免疫组化和非同位素原位杂交方法检测蕈样肉芽肿（ＭＦ）／Ｓéｚａｒｙ综合征（ＳＳ）皮损部位Ｔｈ１型（ＩＬ－２、ＩＦＮ－γ）和Ｔｈ２型（ＩＬ－４）细胞因子的表达。结果显示８例瘤前期ＭＦ和２例斑块期银屑病皮损表皮和／或真皮浸润的淋巴细胞表达ＩＬ－２和／或ＩＦＮ－γ，未见ＩＬ－４的表达；而２例进展期ＭＦ、２例ＳＳ、２例急性期异位性皮炎，表皮和／或真皮浸润的淋巴细胞表达ＩＬ－４，未见ＩＬ－２、ＩＦＮ－γ的表达。本研究结果表明：具有正常细胞免疫功能的早期ＭＦ表达Ｔｈ１型的细胞因子，而细胞免疫力低下的进展期ＭＦ／ＳＳ则表达Ｔｈ２型的细胞因子。

Immunohistochemistry and non-isotopic in situ hybridization methods were used to detect the expression of Th_1 & Th_2 cell-mediated cytokine profile in skin tisaue sections of MF/SS. The expression of IL-2 or IFN-γ was identified in the infiltrating lymphoid cells of early MF(n=8) and plaque-type psoriasis(n =2). The expression of IL-4 was identified in the infiltrating lymphoid cells of advanced MF (n=2) ,Sezary syndrome (n=2) and acute atopic dermatitis (n=2). The results indicate that early MF associated with normal cellular immunity exhibit a Th_1-type cell-mediated cytokine profile whereas advanced MF/SS with abnormal immunity express a Th_2-type profile. Distinctive Th cell cytokine profiles assosiated with skin lesions of patients with MF/SS may be related to the pathophysiology and clinical presentation in MF/SS.