HPLC测定虫草培养基中腺苷和虫草素的含量

Determination of adenosine and cordycepin in culture medium of Cordyceps militaris by HPLC

目的 测定虫草培养基中腺苷和虫草素的含量。方法 采用HPLC法，色谱柱为Waters Nova—Pak C18柱（300mm×3．9mm，4μm）；流动相为水-甲醇-醋酸（185：14：1）；流速1．0ml·min^-1，检测波长260nm。结果 腺苷的线性范围为2．5．50．0μg·ml^-1（r=0．9999），回收率为104．5％，RSD=1．32％；虫草素的线性范围为6．5～130．0μg·ml^-1r=0．9999），回收率为106．1％，RSD=1．46％。结论 该方法灵敏、快速、准确，适用于虫草培养基中腺苷和虫草素的检测。

OBJECTIVE To establish an HPLC method for determining the contents of adenosine and cordycepin in Cordyceps militaris. METHODS The column was Waters Nova - Pak C18 （ 3.9 mm × 300 mm,4 μm）, and mobile phase was water - methanol - acetic acid（ 185 ： 14 ： 1 ） with flow rate of 1.0 ml. min-1 and detection wavelength of 260 nm. RESULTS The linear range was 2. 5 - 50.0 μg·ml^-1 （ r = 0.9999 ） for adenosine and 6.5 - 130.0 μg· ml^ -1 （ r = 0. 9999 ） for cordyecepin, respectively. The average recovery of adenosine was 104.5% with RSD of 1. 316% ; and that of cordycepin was 106.1% with RSD of 1.46%. CONCLUSION This method is rapid and accurate. It used to determine the adenosine and cordycepin in culture medium with satisfactory results.