人鼻咽上皮裸小鼠移植及诱癌实验中的EB病毒DNA检测

THE EB VIRUS DETECTION BY PCR IN EXPERIMENTAL CARCINOGENESIS OF HUMAN NASOPHARYNGEAL EPITHELIA TRANSPLANTED INTO NUDE MICE

人鼻咽组织移植于２０只裸小鼠，分三组再分别多次以ＥＢＶ（７头）、ＥＢＶ＋ＴＰＡ（５头）或生理盐水（对照８头）皮下注射，诱癌后处死，将移植物作常规病理切片观察，并同时提取ＤＮＡ为模板，以ＥＢ病毒ＢａｍＨＩＷ片段特异性引物作ＰＣＲ检测ＥＢ病毒。结果显示：ＥＢＶ组与ＥＢＶ＋ＴＰＡ组中，７只可见鼻咽组织移植物发生癌前或癌变（原位癌、早期浸润癌各１只），而对照组均未见上述改变，两组比较差别有显著意义（０．０１＜Ｐ＜０．０５）；无形态变化的受试动物中ＩＢ病毒阳性２只，阴性１１只；有癌前病变和癌变的动物中，阳性５只，阴性２只，两组差别有显著性（０．０１＜Ｐ＜０．０５），提示观察到的病变与ＥＨ病毒感染有关。

The nude mice with transplants of human nasopharyngeal epithelia(NE)were repeatedly injected subdermally by EBV, EBV+TPA or distillted water, respectively.Transplants were sectioned finally and HE stained, DNAs were extracted for PCRs using the primers specific for EBV DNA BamHI W fragment.The results showed that EBV or EBV+TPA induced not only precancerious changes(5/12),but also carcinomas(2/12)of transplanted NE, they were not found in the control animals.Of 20 cases,2 were positive and 11 negative of EBV PCR in non-change group,however,5 positive and 2 negative in precancerious and cancerious group.There were significant differences between these two groups(0.01<P<0.05),and all carcinomas were EBV positive.It is suggested that the morphological changes be closely related to EBV infection.