AGL提取物总黄酮的部分药效学研究

Pharmacodynamic studies on the extraction of AGL flavonoids

目的:探讨碱-酸法提取的AGL总黄酮的毒性及其对SD大鼠血压及兔动脉粥样硬化动物模型的影响。方法:(1)选用SD大鼠,静脉推注AGL总黄酮(1 mg/kg,2 mg/kg),观察血压波动变化。(2)选用新西兰兔,高脂饲料喂养形成动脉粥样硬化动物模型,灌胃给予AGL总黄酮(5 mg/kg),观察血清总胆固醇(TC)、低密度脂蛋白-胆固醇(LDL-C)、高密度脂蛋白-胆固醇(HDL-C)、甘油三酯(TG)、C反应蛋白(CRP)及主动脉的病理学变化。结果:(1)小鼠对AGL总黄酮最大耐受剂量为6 g/kg。(2)AGL总黄酮(1 mg/kg,2 mg/kg)对SD大鼠血压的影响差异无统计学意义(P>0.05)。(3)AGL总黄酮(5 mg/kg)对高脂饲料喂养形成的兔动脉粥样硬化动物模型的影响:AGL总黄酮组血清TC、LDL、HDL水平与模型组比较,差异无统计学意义(P>0.05);AGL总黄酮组血清TG、CRP水平虽有升高,但与模型组比较差异无统计学意义(P>0.05)。病理学检查结果表明,主动脉内膜可见泡沫细胞、粉红色无定性物质。结论:用碱-酸法提取的AGL总黄酮对大鼠血压和动脉粥样硬化动物模型无显著影响。

Objective： To investigate the acute toxicity of AGL flavonoids （extracted by the basic-acid method） and its effect on blood pressure and arteriosclerosis animal models. Methods： （1） Changes in blood pressure were observed by intravenous injections of AGL flavonoids （1 mg/kg, 2 mg/kg） in rats. （2） Experimental arteriosclerosis animal model were established in New Zealand albino rabbits by hyperlipoidal food, then AGL flavonoids were （5 mg/kg） given orally; Blood samples were collected to assess serum TC, LDL, TG, HDL , CRP and dimension of aortic changes in the histopathology. Results. （1）AGL flavonoids＇ maximum tolerance dose was 6 g/kg. （2） AGL Flavonoids showed no significant changes on blood pressure （P〉0.05）. （3） Compared with the hyperlipoidal food group, AGL flavonoids had no significant change in serum TC, LDL, HDL, but serum TG, CRP is high （P〉0.05）. Foam cells and atherosclerotic plaques were observed in aortic intima in the AGL flavonoids group. Conclusions. It is concluded that AGL flavonoids extracted by the basic-acid method possess no marked effects on blood pressure and arteriosclerosis models in rabbit.