新疆博州蒙古族人群胰岛素受体基因与高血压病的相关性

Association of the INSR gene with essential hypertension in Xinjiang Mongol group

目的:研究蒙古族胰岛素受体(INSR)基因第8外显子NsiⅠ多态性同原发性高血压的关系。方法:选取蒙古族原发性高血压患者84例(高血压组),测定身高、体重、收缩压、舒张压、空腹血糖、血浆胆固醇、甘油三酯。另选取199例健康人群(对照组)进行对照研究。提取DNA,采用链式反应-限制性片段长度多态性(PCR-RFLP)技术测定INSR基因第8外显子NsiⅠ多态性。结果:高血压组与对照组INSR基因第8外显子基因型频率及等位基因频率分布无统计学差异。对照组男性和女性分别进行比较发现,基因型N1N1、N1N2分布在原发性高血压和正常人群中无统计学差异,男性分别为75.0%、25.0%和74.7%、25.3%(P>0.05),女性分别为78.8%、21.2%和75.8%、24.2%(P>0.05);两组男性等位基因频率N1、N2分布分别为87.5%、12.5%和87.3%、12.7%(P>0.05),女性为89.4%、10.6%和87.9%、12.1%,差异亦无统计学意义(P>0.05),同时基因型间血压、血糖及血脂对比差异亦无统计学意义。结论:INSR基因第8外显子NsiⅠ多态性可能与新疆博尔塔拉蒙古自治州蒙古族原发性高血压无关。

Objective： To investigate association of the polymorphism of insulin receptor gene （INSR） exon 8 with essential hypertension in Mongol population in Boertala Mongol Autonomous Prefecture of Xinjiang. Methods： The study covered essential hypertensive and normotensive Mongol subjects in Boertala Mongol Autonomous Prefecture of Xinjiang. Body height and weight, systolic blood pressure and diastolic blood pressure were recorded. Fasting plasma glucose, triglyceride, and cholesterol were measured. Genome DNA was ＇extracted from white blood cells. The segment of INSR was amplified from DNA by polymerase chain reaction（PCR）. The PCR products were digested by restriction endonuclease. Rusults： The distributions of gentypes and alleles for INSR exon 8 did not differ significantly between essential hypertensive subjects and normotensive controls. There was no significant difference in genotypes and alleles between male （or female） essential hypertensive group and normotensive group. Gentypes of N1N1, N1N2 were 75%, 25% and 74.7%, 25. 3% in male between essential hypertensive subjects and normotensive controls （P〉0.05）. Those were 78.8%, 21.2% and 75.8%, 24.2% in famale between essential hypertensive subjects and normotensivecontrols （P〉0.05）. The frequencies of N1 and N2 were 87.5%, 12.5% and 87.3%, 13.7% in male between essential hypertensive subjects and normotensive controls （P〉0.05）. Those were 89.4%, 10.6 % and 87.9%, 12.1% in famale between essential hypertensive subjects and normotensive controls （P〉0.05）. There was no association between INSR exon 8 genotypes and age, BMI, Glu, Triglyceride, Cholesterol, systolic blood pressure and diastolic blood pressure. Conclusions：No evidence was found to suggest an association of the insulin receptor exon 8 NsiⅠpolymorphism gene with essential hypertension in Mongol population in Boertala Mongol Autonomous Prefecture of Xinjiang.