摘要
目的探讨川芎嗪对HSC-T6细胞Smad蛋白细胞内转位的影响。方法在HSC-T6细胞培养皿中加入10-5mol/L川芎嗪培养2h,然后采用免疫荧光法检测HSC-T6细胞内Smad-2和Smad-4蛋白细胞内转位。结果经过川芎嗪作用2h后,未发现Smad-2和Smad-4蛋白在HSC-T6细胞内出现转位。结论川芎嗪作用于细胞时可能阻断TGF-β/Smad的细胞内信号转导,这可能是其抑制HSC细胞增殖的重要机制之一。
Objective To investigate the effect of ligustrazine on the intracellular translocation of Smad protein in HSC - T6 cell line. Methods HSC - T6 cell was cultured with ligustrazine at the dose of 10^-5 mol/L in the culturing dish for 2 hours. After the culturing, the translocation of Smad - 2 and Smad - 4 proteins was detected by immunofluorescence assay. Results There was no evidence of the translocation of Smad - 2 and Smad - 4 proteins in HSC - T6 cell after the culturing. Conclusion Ligustrazine can block the signal pathway of TGF -β/Smad, which may be one of its important mechanisms of inhibiting the proliferation of HSC - TS cell.
出处
《中药新药与临床药理》
CAS
CSCD
2006年第5期320-322,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广东省自然科学基金项目(编号000347)
