HPLC法测定枳实药材中橙皮苷与柚皮苷的含量

Determination of Naringin and Hesperidin in Fructus Aurantii Immaturus by HPLC

目的测定枳实药材中橙皮苷与柚皮苷的含量,为其质量评价提供科学依据。方法采用RP-HPLC法,在同一色谱条件下测定不同批次枳实中橙皮苷和柚皮苷的含量。色谱柱:Merck-LichrospherRP-C18(4.6mm×250mm,5μm);流动相:乙腈-水(23∶77);流速:1.0mL/min;检测波长:283nm,柱温30℃。结果枳实中柚皮苷的含量范围在4.65～12.50mg/g,橙皮苷的含量范围在1.60～6.75mg/g。结论本实验建立的方法具有专属、简便、准确、可重复的特点,可用于枳实药材的质量控制。

Objective To establish a method for the content determination of naringin and hesperidin in Fructus Aurantii Immaturus. Methods HPLC was performed on the Merck-Lichrospher RP-C18 column（4.6 ×250 mm, 5 μm）, CH3CN - H2O （23 ： 77） served as mobile phase and the detection wavelength was at 283 nm, the column temperature at 30℃ and the flow rate being 1.0 mL/min. Results The content of naringin ranged from 4.65 to 12. 50 mg/g and that of hesperidin from 1.60 to 6.75 mg/g. Conclusion This method is simple, sensitive, specific and can be used for the quality control of Fructus Aurantii Immaturus.