荧光探针定量法检测小儿肺炎支原体临床应用研究

INQUIRY INTO THE QUATITATIVE ANALYSIS TECHNIQUE OF THE FLUORESCENCE PROBE TO PAY THE CLINICAL APPLICATION RESEARCH IN THE MYCOPLASMAPNUEMONICE OF THE CHILD PNEUMONIA

目的:探讨荧光探针定量(FQ-PCR)法检测小儿肺炎支原体的临床应用价值。方法:用荧光探针定量(FQ-PCR)法对小儿肺炎支原体DNA进行定量测定。同时用ELISA法MP-IgM(1周内、1周后)进行比较。结果:150例受检患儿中,FQ-PCR法阳性57例,阳性率占38%,DNA平均拷贝数4.65×106;1周内MP-IgM阳性15例,占10%;1周后MP-IgM阳性42例,占28%。FQ-PCR与1周后MP-IgM之间差异有显著性,X2=3.39(P<0.05),FQ-PCR与1周内MP-IgM有显著差异X2=32.27(P<0.005),1周之内与1周之后MP-IgM之间有显著性差异X2=13.91(P<0.005)。结论:应用FQ-PCR技术具有特异性强,灵敏度高,是早期快速诊断小儿肺炎支原体感染的可靠方法,值得推广使用。

Objictive： To discuss the clinical value of using the Fluorescent Probe Quantitative Method （ FQ - PRC） to check and measure the Mycoplasma of the Child Pneumonia. Methods ： Use the FQ - PRC Quantitative Method to do the quantitative estimation. At the same time, use MP - IgM of the ELISA Method （during a week, and after a week） to do the same quantitative estimation. Compare the results of the two methods. Results ： In 150 checked sick children, using the FQ - PRC to check, 57 of them are positive. The positive rate is 38%, and the average copy number of DNA is 4.65 ×10^6; At the same time, using the ELISA to check, 15 of the 150 children are MP -IgM positive during a week, and the positive rate is 10%. A week later, 42 of the 150 children are positive, and the positive rate is 28%. There is an obvious distinction between the result of FQ - PRC Quantitative Method and the result of MP - IgM （ after a week）, X2 = 3. 39 （P 〈 0. 05 ） ; also a obvious distinction between the result of FQ - PRC and the result of MP - IgM （ during a week）, X2 = 32. 27 （ P 〈 0. 005 ） and an obvious distinction between the results of MP - IgM during a week and after a week X2 = 13.91 （ p 〈 0. 005 ）. Conclusion ： the FQ - PRC Quantitative Method is sensitive and accurate. It＇s a good way to get a rapid diagnosis in the early stage of the mycoplasma infection of the Child Pneumonia. It should be popularized and applied widely in clinical work.