低温台温度场分布的理论和实验研究

Theoretical and Experimental Study of Temperature Distribution on a Cryostage

低温显微镜是生物材料低温保存研究中的重要工具之一。低温台上热电偶的布置位置和样品的体积都能影响测温的准确性。对低温台温度场的分布情况进行了数值模拟,并通过实验验证。分析结果表明可以利用布置在盖玻片上表面的热电偶测温数据推算实验样品层的温度,样品层温度在水平方向上是基本均匀的。

Cryomicroscope is an important tool in the study of cryopreservation of biomaterials. The accuracy of tem- perature measurement is influenced by the location of the thermocouple and the volume of the sample. The temperature distribution on the cryostage was numerically simulated and verified with experiments. The results show that the temperature of the sample can be estimated from the measured value of the thermocouple placed on top of the cover glass, and the planar temperature distribution of the cryostage is approximately uniform.