O/W型微乳的制备及其对皮肤刺激性的检测

Preparation of O/W microemulsion and measurement of skin irritation of microemulsion

通过拟三元相图微乳区大小筛选微乳制备组分的较佳配比,电导率法检测微乳的类型,研究了以十四酸异丙酯、十六酸异丙酯、大豆油为油相,吐温80和卵磷脂质量比以及乙醇、1,2-丙二醇为助表面活性剂对微乳形成区域的影响。结果表明:选用十六酸异丙酯作油相,吐温80和卵磷脂质量比为2∶1作混合表面活性剂,乙醇为助表面活性剂时能形成较大的O/W型微乳区。经动物实验证实能够满足药用无毒、无刺激性要求,适合作为透皮吸收药物载体。

Adopting isopropyl myristate, isopropyl palmitate, and soybean oil as different oil phase materials, Tween 80 blended with lecithin as surfactant as well as ethanol and 1,2 - propylene glycol as different co - surfactant, O/W microemulsions were prepared respectively. In accordance with the area of the microemulsion regions in the pseudo ternary phase diagram, the effect of the ingredients on the formation of microemulsion region was studied and the kind and blending ratio of the ingredients were screened and identified. The type of the microemulsion was identified by measurement of the electrical conductivity of the microemulsion. Experimental results showed that, in order to form rather big area of the microemulsion region, isopropyl palmitate is to be used as oil phase; the optimal mass ratio of Tween 80 to lecithin is 2 ： 1 ; while ethanol is to be used as co - surfactant. Through animal skin test, the O/W microemulsion is confirmed satisfactory in aspects of non - poisonous and non - irritation. Such O/W microemulsion is suitable for using as carrier for skin penetration topical pharmaceuticals.