水稻“9311”突变体筛选和突变体库构建

The Screening of Mutants and Construction of Mutant Population for Cultivar "9311" in Rice(Oryza sativa L.)

利用γ射线和EMS溶液诱变处理籼稻“9311”种子,经过M2筛选和M3重复鉴定,分别获得465份和210份(共675份)叶、茎、穗和根等性状变异的突变体,突变频率为5.62%。γ射线诱变群体的变异范围要大于EMS诱变群体,突变频率也较高,但紫色叶鞘和叶片类病斑等少数突变类型只在EMS诱变群体中出现。新构建的突变体库将有助于进一步开展水稻功能基因的研究。

Rice has become an established model system in the last decade and the organism chosen in the study of the cereal genomes. In order to find more mutants in the same genetic background, the seeds of indica rice cultivar 9311 were treated with γ-rays or EMS （ethyl methan sulfonate）. Some mutants with the variations of leaf, stalk, panicle or root traits were found in the progenies, which the mutation frequency was 5.62 %. Four hundred and sixty-five out of the 675 mutants were induced by γ-rays, and the other 210 mutants by EMS. Comparing with the EMS mutagenesis population, more mutants were produced in γ-rays mutagenesis population. But some mutants, such as the purple leaf sheath mutant and lesion resembling disease mutant, were only found in EMS mutagenesis population. The mutant library created will be helpful for gene identification and gene functional analysis.