IBVS1基因RT－PCR产物的分子克隆

RESEARCH ON THE MOLECULAR CLONING OF THE RT-PCR PRODUCTS OF IBV S1 GENE

报道了在两条引物的５′端加有不同酶切位点的ＩＢＶＤ４１株Ｓ１基因ＰＣＲ产物与ｐＵＣ１８进行分子克隆的研究情况。采用自己改进的微量基因克隆的方法，经回收的插入片段的分子量大小及ＨａｅⅢ酶切分析。

The S1 gene of IBV strain D41 was isolated by RT-PCR using two primers (oligo 5′ and oligo 3′) which were synthesized with a HindⅢ cleavage site added to the 5′ end of oligo 5′ and a BamHI site added to the 5′ end of oligo 3′. The gene was cloned into pUC18. By checking the DNA molecular weight and digestion of the recovered insert with HaeⅢ, it was comfirmed that two recombinant plasmids were obtained.