摘要
目的 研究白介素2α受体（IL-2Rα）在人视网膜色素上皮（RPE）细胞中的表达及白介素2（IL-2）对RPE细胞增生的影响。方法收集培养的2～4代的人胚胎RPE细胞，应用RT-PCR技术，用IL-2α受体特异引物对IL-2α受体进行检测，应用荧光激活细胞分类技术检测IL-2的结合。用抗CD25抗体的免疫荧光染色法识别IL-2α受体的表达，通过^3H摄取技术评估重组IL-2对RPE细胞增生的影响。结果 RPE细胞可表达IL-2Rα mRNA，免疫荧光染色和IL-2结合试验也可表明IL-2α受体的表达，高浓度的IL-2可诱导RPE细胞的增生（P〈0．05）。结论 培养的RPE细胞能够表达IL-2α受体，重组IL-2可促进RPE细胞的增生。
Objective To investigate the expression of interleukin 2 receptor alpha （IL-2Rα） in human retinal pigment epithelial cell （RPE） and the effect of IL-2 on RPE proliferation. Methods Passage 2 - 4 human fetal RPE cells were used in the experiment. RT-PCR was performed with the specific primer for IL-2Rα, IL-2 binding was assayed by fluorescence-activated cell sorting. Immunofluorescent staining was applied to identify the receptor expression using anti-CD25. The effect of recombinant IL-2 on RPE cell proliferation was determined by^3H uptake. Results RPE cells expressed IL-2Rα mRNA. The expression of IL-2 receptor α was also revealed by immunofluorescent staining and IL-2 binding. IL-2 induced cell proliferation at the higher concentrations of IL-2 （ P 〈0. 05 ）. Conclusion Cultured human RPE cells express IL-2α receptor. Recombinant IL-2 enhances RPE cell proliferation.
出处
《眼科研究》
CSCD
北大核心
2006年第5期452-456,共5页
Chinese Ophthalmic Research
