自由落体致大鼠脑损伤后血脑屏障变化的研究

Disruption of Blood-brain Barrier Permeability after Brain Trauma in Rats

目的用IgG免疫组化染色方法研究大鼠自由落体脑损伤后血脑屏障的变化。方法复制大鼠自由落体脑损伤模型,在外伤后1h、6h、24h及7d时取脑组织,分别进行光镜、电镜检查,同时用IgG免疫组化染色方法观察血脑屏障的变化,并对免疫组化结果进行半定量分析。结果大鼠自由落体脑损伤后,损伤部位出现片状出血及明显水肿,电镜观察显示有毛细血管内皮细胞损伤及星形细胞肿胀,免疫组化染色显示IgG在脑损伤后1h即有漏出,6h漏出最严重,持续至24h,7d时降低。结论大鼠脑损伤后出现血脑屏障通透性改变;IgG免疫组化染色是研究血脑屏障功能变化简单、敏感的方法。

Objective To investigate the disruption of blood-brain barrier （BBB） after brain trauma in rats using IgG immunohistochemical staining. Methods An impact-acceleration head injury model was established with rats. Histological changes of rats＇ brains were observed by HE staining and light and electron microscopes at 1 h, 6 h, 24 h and 7 d after injury, and BBB permeability was analyzed semi-quantitatively by IgG immunohistochemical staining at the same time points. Results The spot bleeding and brain edema was present in the damage region after brain trauma and endothelial cell damage and astrocyte swelling could be found under electron microscope. Tile extravasations of IgG was detected in the injured hemisphere of ratsat 1 h, and achieved the peak at 6 h, remained a high level up 1o 24 h, and decreased at 7th d. Conclusion The disruption of BBB function occurs after Drain trauma in rats, and detection of IgG extravasations tested by immunohistochemical staining is a simple and sensitive way to investigate BBB permeability.