自体血三次注入法建立脑出血大鼠模型

An Intracerebral Hemorrhage Model Established by Autologous Blood Injection Three Times in Rat

目的建立稳定的脑出血大鼠模型,并观察动物的行为学改变、脑水肿及组织结构变化。方法取大鼠尾动脉血100μl,分3次注入大脑尾状核,观察动物的行为学改变,在不同时间点处死动物,观察脑组织水肿及组织学变化。结果模型成功率75%,出血后6h出现行为学改变,持续4～5d,7d时基本恢复正常;脑组织含水量于出血后6h增加,48～72h达高峰,7d时基本恢复正常。脑组织于出血早期表现为神经细胞水肿,后期表现为胶质细胞增生。结论此模型死亡率低,成功率高,血肿稳定,行为学改变明显,病理学改变符合临床。

Objective To establish an animal model of intracerebral hemorrhage （ICH） in rat and to observe the changes in behavior, brain edema and tissue structure during absorption of the hematoma. Methods An animal model of ICH was established by stereotactically infusing 100 μl （the first time 20 μl, the second time 40 μl, the third time 40 μl） of autologous caudal artery blood into caudate nucleus in the rats. The behavior of rats with ICH was assessed by Bederson score, beam-walking lest, bilateral forepaws grasp and measurement of forelimb placing. The brain edema and the change in histology were observed. Results The successful ratio of this model was 75%. There were significant behavioral changes and brain edema at 6 h after the ICH in rats of the experimental group compared to those of the control group. The worse behavioral changes and brain edema was at 48-72 h after hemorrhage. In the earlier period, the nerve cells were swelling. A lot of glial cells appeared around the hematoma in the late period. Conclusion This ICH model is reproducible, and the obvious behavioral change and histological changes are similar to clinical patient with ICH.