用基因芯片表达谱筛选恶性黑色素瘤差异基因的研究

Study of Screening Differential Genes of Malignant Melanoma Using Gene Chip Expression Profiles

目的用基因芯片表达谱筛选出恶性黑色素瘤的差异基因。方法用AgilentHuman1AOligoDNA芯片对恶性黑色素瘤和色素痣组织中基因表达情况进行检测,提取总RNA,进行荧光标记探针、杂交、洗涤后分析。结果恶性黑色素瘤和正常色素痣组织共有1596个差异表达基因,其中上调基因733个,下调基因863个。结论基因芯片技术可筛选出恶性黑色素瘤相关基因。

Objective To identify differential genes of malignant melanoma using gene chip expression profiles. Methods Agilent Human 1A OligoDNA was employed to find out difference in gene expression between malignant melanoma and nevus. The total RNA was isolated from two type tissues, labled the fluorescent to the probe, hybridized, washed and analyzed. Results Among the 21073 target genes, 1596 genes were differentially expressed in malignant melanoma, including 733 genes up-regulated, and 863 down-regulated. Conclusion The gene chip technique can screen genes that may be specifically expressed in malignant melanoma.