艾炷灸贴预处理对AA大鼠足肿胀率及应激激素的影响

Effect of Dressing and Moxibustion-pretreatment on Local Feet Swelling and Stress Hormone in Hypothalami of Rats with Adjuvant Arthritis at Early and Secondary Stages

目的观察艾炷灸贴预处理对随后佐剂性关节炎(AA)大鼠早期和继发期足肿胀率及下丘脑应激激素的影响。方法将40只Wistar大鼠随机分为正常组、模型早期组和继发组、预灸贴早期组和继发组共5组,于造模前用中药膜剂贴敷大鼠大椎穴后加艾灸,观察其对随后AA大鼠下丘脑促肾上腺皮质激素释放激素(CRH)、β内啡肽(βEP)、神经肽Y(NPY)及足肿胀率的影响。结果造模后,模型早期组和继发组大鼠的右足肿胀率明显升高(P<0.01),预灸贴各组较同期模型组降低(P<0.05～0.01);下丘脑CRH水平造模后升高(P<0.05～0.01),但预灸贴早期组较同期模型组有下调趋势,预灸贴继发组下降(P<0.05);βEP水平造模后早期组明显升高(P<0.01),继发组变化不大;与同期模型组相比,预灸贴早期组βEP明显下降,而继发组βEP却明显升高(均P<0.01);下丘脑NPY水平造模后升高(P<0.05～0.01),预灸贴继发组NPY较同期模型组下降(P<0.05)。结论艾炷灸贴预处理可减轻随后AA大鼠足肿胀程度,机理可能与其调节下丘脑CRH、βEP及NPY水平有关。

Objective To observe the effect of dressing and moxibustion-pretreatment on the local joint swelling and stress hormone in hypothalami of rats with adjuvant arthritis （AA） at early and secondary stages. Methods Forty Wistar rats were randomly divided into 5 groups： normal group, early and secondary model groups, early and secondary pre-dressing and moxibustion （PDM） groups. The dressing with Chinese herb and moxibustion was stuck on Dazhui point （GV14） before the AA model established. The effects of dressing and moxibustion-pretreatment on the feet swelling and cortlcotropin-releasing-hormone （CRH）, beta-endorphin （β-EP） and neuropeptide-Y （NPY） in hypothalami were observed. Results The right feet swelling rate at early and secondary stages obviously increased after modeling （P 〈0.01 ）, and it became lower in early and secondary PDM groups than in model groups at the same phases （ P 〈0.05-0.01 ）. The level of hypothalamic CRH was higher after modeling （ P 〈0.05-0.01 ）, compared with early model group, it had a tendency to going down in early PDM group, moreover, in secondary PDM group the level was similar with the normal group. The level of hypothalamic β-EP increased significantly in early model group （ P 〈0.01 ）, and lightly changed in secondary model group, it decreased in early PDM group but increased in secondary PDM group, compared with model groups at the same stages （P 〈0.05）. The level of hypothalamic NPY increased significantly after modeling, and it declined in the secondary PDM group （ P 〈0.05）. Conclusion Dressing and moxibustion-pretreatment can relief feet swelling of AA rats, which may be related with its regulative effect on the level of hypothalamic CRH, β-EP and NPY.