粒细胞集落刺激因子对CD34^+骨髓干细胞在心肌内归巢的影响

Effects of granulocyte colony-stimulating factor on CD34^+ bone marrow stem cell homing to infarcted myocar- dium

目的观察粒细胞集落刺激因子(G-CSF)对CD34+骨髓干细胞(BMSC)在心肌内归巢的影响.方法成龄杂种犬17只,随机分4组.A组5只接受10μg/(kg.d),5d皮下注射和实验性心梗;B组4只接受10μg/(kg.d),5d皮下注射和假手术;C组4只接受10μg/(kg.d),5d生理盐水皮下注射和实验性心梗;D组4只接受10μg/(kg.d),5d生理盐水皮下注射和假手术,对每只动物股动脉插管至主动脉根部,左颈浅静脉插管至冠状静脉窦,分别于造型前后抽血5mL(抽复管),裂解红细胞,分离单个核细胞,流式细胞仪测定CD34+细胞数,数据分析用SPSS10.0统计软件包.结果4个实验组CD34+BMSC心肌内归巢数分别为(183.0±9.2)×104个/L,(71.1±9.2)×104个/L,(82.3±9.5)×103个/L和(78.5±9.8)×103/L,除C,D组之间差别无统计学意义(P=0.912)外,其余各组间均有统计学意义(均为P=0.000).结论G-CSF不仅能动员BMSC大量释放入外周血,同时还能增加CD34+BMSC在心肌内的归巢.

AIM： To explore the possible effects of granulocyte colony-stimulating factor （G-CSF） on the homing of CD34^＋ bone marrow stem cell （BMSC） to myocardium. METHODS： Seventeen adult mongrel dogs were randomly assigned to 4 groups. Group 1 （G-CSF and MI, n =5） were treated with 10μg/（kg·d） ×5 d of G-CSF injection sc and then myocardial infract operation; group 2 （G-CSF and sham operation, n =4） , treated with 10 μg/（kg · d） ×5 d of G-CSF injection sc and sham operation; group 3 （non-G-CSF and MI, n =4） were injected with 10 μg/ （kg · d） ×5 d sc normal saline and underwent myocardial infarct operation; group 4 （non-G-CSF and sham operation, n=4） were injected with 10 μg/（kg · d） ×5 d sc normal saline and get sham operation. Thoracotomy was performed on the left thorax of each animal and left anterior descending artery was exposed and separated about 1 cm. In group 1 and 3 the separated arteries were occluded for 3 h and then re-opened, while the other 2 groups were not treated in this way. Every dogs received catheterizing in right femoral artery into the root of aorta arch and a top-modified pigtail catheter cannulating through left jugular superficial vein into coronary venous sinus. 5 mL blood from each animal aorta and coronary sinus were harvested for two times, in which erythrocytes were lysed. Mononuclear cells were separated with lymphocyte separating solution and CD34^＋ BMSC ＂were clarified and counted by flow cytometry. Data were calculated and managed with SPSS 10.0 statistical package. RESULTS： The amount of the CD34^＋ stem cells homing to myocardium in each group were （183.0＋9.2） ×10^4/L, （71. 1 ＋9.2） ×10^4/L, （82.3 ＋ 9.5） ×10^3/L and （78.5 ＋9.8） × 10^3/L, respectively. We found after comparing the data of each group that the difference among every group was very significant （ P = 0.000, respectively） except between group 3 and 4 （ P = 0. 912）. CONCLUSION： G-CSF could not only vastly mobilize the BMSC into periphery blood, but also could increase the CD34^＋ stem cells homing to myocardium independent of myocardial infarct.