MMP-9和PEDF在胃癌侵袭转移中的作用

Expressions of MMP-9 and PEDF and their correlation with tumor invasion and lymph node metastasis in gastric carcinoma

目的探讨基质金属蛋白酶9(matrix metalloproteinase 9,MMP-9)和色素上皮源性因子(pigmentepithelium-derived factor,PEDF)基因表达在胃癌侵袭转移中的作用,为防治胃癌转移提供理论依据。方法应用免疫组织化学法检测82例人体胃癌组织标本中胃癌细胞内MMP-9和PEDF的表达;统计学分析两种分子标记物与胃癌侵袭转移关系。结果胃癌组织中的MMP-9和PEDF表达率分别约为70%和43%,MMP-9高表达和PEDF低表达与浸润深度、淋巴管转移和远处转移呈正相关(P<0.05);MMP-9与PEDF表达呈负相关(P<0.01)。结论MMP-9在胃癌侵袭转移中起促进作用,PEDF可抑制胃癌的进展,PEDF低表达可能通过上调MMP-9的表达从而促进胃癌的侵袭转移。

Objective To investigate the expressions of Matrix Metalloproteinase 9 （MMP- 9） and Pigment epithelium - derived factor （PEDF） and their correlation with ttanor invasion and metastasis in human gastric carcinoms, Methods Immunohistochemistry method was used to detect the expressions of MMP- 9 and PEDF in 82 eases of gastric carcinoma tissue samples. The relationship between the molecular markers and tumor invasion and metastasis were analyzed. Results The expression rates of MMP - 9 and PEDF were 70% and 43% respectively in gastric carcinoma patients. MMP - 9 expression was related to depth of invasion and lymph node metastasis as well as distant metastasis. Low PEDF protein expression was significantly associated with tumor- increased penetration through the gastric wall, lymph node and distant metastasis. There was negative correlation between the expression of MMP- 9 and PEDF. Conclusion MMP- 9 can accelerate the development and progression of gastric carcinoma, while PEDF play a negative role in tumor progression. The low expression of PEDF may promote the tumor invasion by upregulating the expression of MMP- 9,