一些杀菌剂和抗菌素对根癌菌和根癌抑制菌的作用

Effect of Some Bacteriocides and Antibiotics on Agrobacterium tumefaciens and Anti-Gall Bacteria

采用抑菌留法测定了6种杀菌剂和抗菌素对不同生物型根瘤上壤杆菌及其抑制菌的作用。结果表明，福美双对生物Ⅲ型根癌菌所有菌株都有较强抑制作用，抑菌圈直径（DBA）最大达23．2mm，对Ⅰ，Ⅱ型的个别菌株也有轻微抑制作用；多菌灵、乙磷铝和加瑞农对3种生物型的根癌菌无抑制作用；青霉素钠对Ⅰ，Ⅱ型根癌菌无抑制作用，对Ⅲ型抑制作用较强，最大DBA为27．0mm。硫酸链霉素对Ⅰ，Ⅱ，Ⅲ型根瘤菌抑制作用显著，DBA最大值达40．8mm。对于根癌抑制菌，福美双在＜1000μg·g-1时．对K84和E26无作用，对HLB－2作用微弱，DBA为11．7mm。多菌灵、乙磷铝对3种根癌抑制菌均无作用。加瑞农对K84无作用，对E26和HLB－2作用轻微，最大DBA分别为13．2mm和10．2mm。青霉素钠对K84，HLB－2无作用，对E26的最大DBA达375mm。硫酸链霉素对HLB－2和E26无作用，对K84的最大DBA达26．2mm.

This study reported the bacteriostasis of some bacteriocides and antibiotics on different bio type of Agrobacterium causing crown gall and its inhibiting bacteria. The results showed the obvious bacteriostasis of thiram and penicillin on the crown gall pathogens of biotype Ⅲ, their maximum. mean diameter of bright area (DBA) were 23. 2 mm and 27. 0 mm respectively. Thiram showed slight inhibition on some strains of biotype Ⅰ and Ⅱ of A. tumefaciens, but penicillin expressed no effects on these two biotypes. There were no bacteriostasis of carbendazim, phosethyl-Al, and kasumin-bordeaux on the above three biotypes. Streptomycin expressed strong inhibition on the pathogenic bacteria of biotype Ⅰ,Ⅱ, and Ⅲ. The maximum. average DBA was 40. 8 mm. For anti-gall strains, K84 and E26 were not sensitive to thiram, and the DBA of thiram (concentration <1 000 μ.g-1) on HLB-2 was 11.7 mm. Carbendazim andphosethyl-Al showed no effects on K84, E26, and HLB-2. Kasumin-bordeaux showed very weak inhibition on E26 and HLB-2, their maximum. mean DBA were 13. 2 mm and 10.2 mm, respectively, but no effect on K84. Strains E26, but not K84 and HLB-2, was sensitive to penicillin. The maximum. average DBA were 37. 5 mm. Streptomycin produced maximum. 26. 2 mm DBA on K84, but no effect on HLB-2and E26.