静脉移植物中整合素α5β1的表达及其与再狭窄的关系

Relation of integrin α5β1 expression in human vein grafts to restenosis

目的检测人静脉移植物中整合素α5β1的表达。方法应用免疫荧光组织化学技术对30个静脉移植物再狭窄标本中整合素α5β1和α-smooth musc le actin(α-SMA)的表达进行了检测,用激光共聚焦显微镜拍片,图片用S iliconG raph ics Octane进行处理。结果在正常静脉血管中,整合素α5β1在中膜平滑肌细胞和内皮细胞呈微弱表达;α-SMA在中膜平滑肌细胞表达;在病变静脉血管,整合素α5β1在中膜平滑肌细胞、内膜内皮细胞中的表达呈强阳性,在内膜平滑肌细胞中也有弱表达。α-SMA除在中膜平滑肌细胞表达外,在内膜平滑肌细胞也有表达。结论静脉移植物整合素α5β1在中膜平滑肌细胞、内膜内皮细胞表达显著上调,提示整合素α5β1参与静脉移植物再狭窄的形成。

Objective To detect the expression of integrin α5β1 in restenosed human vein grafts. Methods The expression of integrin α5β1 and α-smooth muscle actin （α-SMA） in 30 resected restenosed human vein grafts （ from Kerfuff clinical hospital of Germany） was detected by confocal immunofluorescence with specific antibodies against α5β1 and α-SMA. Images were processed with Silicon Graphics Octane. Results In normal veins, integrin α5β1 expression was very weak in media smooth muscle ceils and in endothelium, and α-SMA expression was present in media smooth muscle ceils. In the restenosed vein grafts, integrin α5β1 was strongly stained in the media smooth muscle ceils and intimal endothelial ceils, and moderately in the intimal smooth muscle ceils, α- SMA was present in media smooth muscle ceils and in the intimal smooth muscle ceils. Conclusion Our research reveals that integrin α5β1 is significantly upregnifiated in the media smooth muscle ceils and intimal endothelial ceils in the restenosed human vein grafts, suggesting the participation of integrin α5β1 in the restenosis formation of human vein grafts.