化学致癌剂3’-甲基-4-DAB影响SD大鼠肝干细胞的增殖与迁移

Chemical carcinogen 3’-me-DAB affects proliferation and migration of rat hepatic stem cells

目的采用免疫组织化学技术标记SD大鼠肝干细胞中OV6、CD34的表达,探讨肝干细胞与造血干细胞之间的关系。方法用化学致癌剂3’-甲基-4-二甲基氨偶氮苯(3’-methy-4-dimethylaminoazobenzen,3’-me-DAB)喂养SD大鼠4周,取大鼠肝脏,用免疫组化法标记卵圆细胞的特异性标记物OV6与造血干细胞标记物CD34在大鼠肝干细胞中的表达。结果汇管区靠近界板处卵圆细胞同时表达OV6与CD34,阳性细胞成簇状分布,主要集中在赫令氏管周围,并见OV6和CD34阳性细胞向肝小叶内迁移,散在分布于肝细胞索;在肝实质内还分布着大量的表达OV6与CD34阳性的单核样干细胞;在肝实质内还可见多个CD34阳性小集落,集落内或周围也可见少许单核样干细胞。结论3’-me-DAB刺激大鼠肝内卵圆细胞大量增生的同时,还可刺激骨髓造血干细胞增生并向肝内迁移与分化。

Objective To explore the relationship between hepatic stem cells and hematopoietic stem cells by detecting the expression of OV6 and CD34 in rat hepatic oval cells. Methods Healthy SD rat were fed with chemical carcinogen 3＇ -me-DAB for 4 weeks, then the rat hepatic tissue samples were obtained. The rats fed with normal forage served as normal controls. The expressions of OV6 and CD34, specific markers for oval cells and hematopoietic stem cells respectively, in rat hepatic stem cells were detected by immunocytochemical staining （ABC method）. Results The oval cells positive for OV6 and CD34 were found in the limiting plate of portal area, mainly located around the Hering canal. In addition, some oval cells positive for OV6 and CD34 were found migrating to hepatic lobule, scattering in the hepatic cords. A great many mononuclear stem cells positive for OV6 and CD34 were also found in the hepatic parenchyma. Conclusion 3＇ -me-DAB could stimulate the proliferation of hepatic oval cells and hematopoietic stem cells and cause the hematopoietic stem cells derived from bone marrow to migrate to hepatic parenchyma and differentiate.