福建省烟粉虱生物型鉴定

Biotype identification of the populations of Bemisia tabaci in Fujian,China

采用m tDNA分子标记技术以及西葫芦银叶测定方法,对从福建省8个具有代表性地理区域采集的烟粉虱样品进行生物型鉴定.结果表明:8个烟粉虱种群在m tDNA COⅠ基因720 bp片段序列上的所有位点都相同,它们与Texas-B、Israel-B、Ind ia-B、Morocco-B、France-B1、France-B2、Be ijing-B、Argentina-B、Reun ion-B1、Reun ion-B2、Reun ion-B3等11个B型烟粉虱种群在m tDNA COⅠ基因720 bp序列上的差异很小(序列相似性为99.6%-100%),总体上属于同一进化枝;与Morocco-Q、Colomb ia-A1、Colomb ia-A2烟粉虱种群在m tDNA COⅠ基因720 bp序列上差异较大,分别属于不同的进化枝;8个烟粉虱种群均可引起典型的西葫芦银叶症状.说明福建省8个烟粉虱种群均属于B生物型.

The biotype of Bemi, ia tabaci （C, ennadius） （Hemiptera：Aleyrodidae） collected from 8 representative geographical locations in Fujian Province, China was determined by mitochondria cytochrome oxidase Ⅰ （mtCO Ⅰ ） markers and squash silverleaf assay. Of 720 characters examined for CO Ⅰ , 8 populations all had same sequence, which showed little diversity and shared 99.6%- 100% identity with the reference sequences from 11 B-biotype. Three distinct clades were revealed in the mtCO Ⅰ tree constructed. Clade one included all of B. tabaci collections and reference B-biotype populations, clade two and three only consisted of Q-biotype and A-hiotype populations, respectively. Squash silverleaf assay demonstrated that all populations of B. tabaci collected in Fujian Province cound induce typical squash silverleaf. It was concluded that the 8 populations all belonged to B-biotype.