宫颈癌患者血浆中E-钙黏着蛋白基因启动子区的甲基化状态

The Promoter Methylation Status of E-cadherin Gene in Plasma Cervical Cancer Patients

肿瘤细胞可以释放DNA进入患者的血浆/血清中,并可作为无创伤性诊断肿瘤的标记物。采用甲基化特异性聚合酶链式反应(MS-PCR)结合亚硫酸盐测序法对151例宫颈癌患者血浆和对应的30例组织中E-钙黏着蛋白基因启动子区甲基化状态进行检测,并与化学发光法检测患者血清的鳞状上皮癌抗原(SCC)相比较,发现此方法的灵敏度为40.39%,特异性为100%,正确性为49.72%,血浆和组织的符合率为76.67%。宫颈炎、子宫肌瘤和正常人的血浆中均未检测到甲基化状态的存在。随着临床分期和组织学分级的增加,E-钙黏着蛋白基因甲基化的检出率也在逐渐增加,与SCC结果相比,MS-PCR方法在早期和恶性度高的宫颈癌中的诊断效果良好。使用E-钙黏着蛋白基因作为分子标记可以对宫颈癌患者进行无创伤性早期诊断和预后的评估。

Recent evidence suggests that tumor cells may release DNA into the serum and plasma of cancer patients and it can be used as a non-invasive marker for diagnosing cancer. In this study we examine the promoter methylation status of E-cadherin gene in 151 pretreatment plasma samples and 30 paired tumor tissue samples from cervical cancer patients using methylated specific polymerases chain reaction （MS-PCR）. and sequencing. At the same time, chemical luminal immune assay （CLIA） was used to detect the squamous cell carcinoma （SCC） in plasma of cervical cancers. The results show that the sensitivity of the method is 40.39%, the specificity is 100% and the correct rate is 49.72%. The total concordant rate of methylation status between plasma samples and tissue samples was 76.67%. We could not find the methylation status in control groups. The higher of the clinical stage and histological grade is, the higher of the positive rate of methylation in E-cadherin gene in plasma samples. Compared with the results of SCC, MS-PCR is valuable in diagnosing cervical cancer in early stage and high grade. Using the E-cadherin gene as detecting marker we can diagnose and evaluate the effect of treatment to cervical cancers as soon as possible.